Next Generation Sequencing Workshop at the Royal Society of Medicine (London, May 2022): how genomics is on the path to modernizing genetic toxicology.

IF 2.5 4区 医学 Q3 GENETICS & HEREDITY
Mutagenesis Pub Date : 2023-08-24 DOI:10.1093/mutage/gead012
Anthony M Lynch, Thalita B Zanoni, Jesse J Salk, Inigo Martincorena, Robert R Young, Jill Kucab, Charles C Valentine, Carole Yauk, Patricia A Escobar, Kristine L Witt, Roland Frötschl, Simon H Reed, Anne Ashford
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Abstract

The use of error-corrected Next Generation Sequencing (ecNG) to determine mutagenicity has been a subject of growing interest and potentially a disruptive technology that could supplement, and in time, replace current testing paradigms in preclinical safety assessment. Considering this, a Next Generation Sequencing Workshop was held at the Royal Society of Medicine in London in May 2022, supported by the United Kingdom Environmental Mutagen Society (UKEMS) and TwinStrand Biosciences (WA, USA), to discuss progress and future applications of this technology. In this meeting report, the invited speakers provide an overview of the Workshop topics covered and identify future directions for research. In the area of somatic mutagenesis, several speakers reviewed recent progress made with correlating ecNGS to classic in vivo transgenic rodent mutation assays as well as exploring the use of this technology directly in humans and animals, and in complex organoid models. Additionally, ecNGS has been used for detecting off-target effects of gene editing tools and emerging data suggest ecNGS potential to measure clonal expansion of cells carrying mutations in cancer driver genes as an early marker of carcinogenic potential and for direct human biomonitoring. As such, the workshop demonstrated the importance of raising awareness and support for advancing the science of ecNGS for mutagenesis, gene editing, and carcinogenesis research. Furthermore, the potential of this new technology to contribute to advances in drug and product development and improve safety assessment was extensively explored.

英国皇家医学会下一代测序研讨会(伦敦,2022年5月):基因组学如何走上基因毒理学现代化的道路。
使用纠错的下一代测序(ecNG)来确定致突变性一直是人们越来越感兴趣的主题,也是一种潜在的颠覆性技术,可以补充并及时取代临床前安全评估中的现有测试范式。考虑到这一点,2022年5月,在英国环境突变体学会(UKEMS)和TwinStrand Biosciences(WA,USA)的支持下,在伦敦皇家医学会举办了下一代测序研讨会,讨论该技术的进展和未来应用。在本次会议报告中,受邀的发言者概述了研讨会的主题,并确定了未来的研究方向。在体细胞诱变领域,几位发言者回顾了将ecNGS与经典的体内转基因啮齿动物突变分析相关联的最新进展,以及探索该技术直接在人类和动物以及复杂类器官模型中的应用。此外,ecNGS已被用于检测基因编辑工具的脱靶效应,新出现的数据表明ecNGS有可能测量携带癌症驱动基因突变的细胞的克隆扩增,作为致癌潜力的早期标志物,并用于直接的人类生物监测。因此,该研讨会展示了提高认识和支持推进ecNGS科学用于诱变、基因编辑和致癌研究的重要性。此外,这项新技术在促进药物和产品开发进步和改进安全性评估方面的潜力也得到了广泛探索。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Mutagenesis
Mutagenesis 生物-毒理学
CiteScore
5.90
自引率
3.70%
发文量
22
审稿时长
6-12 weeks
期刊介绍: Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.
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