Comparative transcriptome analysis of the effects of friction and exogenous gibberellin on germination in Abrus cantoniensis.

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI:10.1080/15592324.2022.2149113

Zhu Yanxia, Jiang Jianping, Huang Yanfen, Dong Qingsong, Wei Kunhua

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Abstract

The seeds of Abrus cantoniensis (A. cantonensis) have dormancy characteristics with very low germination under natural conditions. In general, its seed dormancy could be broken by friction or soaking with exogenous gibberellins (GA₃). To date, the molecular mechanism underlying the effects of GA₃ and friction on its seed germination is unclear. In this study, we tested the effects of different treatments, including soaking in sterile water (G1), friction (G2), soaking in GA₃ (G3), combined treatment of friction, and GA₃ (G4)) on seed germination. Then, we have investigated the seed transcriptome profiles corresponding to the different treatments by RNA sequencing. The results showed that seed germination was significantly increased by combined treatment with friction and GA₃. RNA-Seq analysis generated 84.80 gigabases (Gb) of sequences. 82,996 out of 121,776 unigenes were annotated. Comparative transcriptome analysis observed that 1,130, 1,097, and 708 unigenes were deferentially expressed in G1 vs. G2, G1 vs. G3, and G1 vs. G4 groups, respectively. Additionally, 20 putatively candidate genes related to seed germination, including CYP78A5, Bg7s, GA-20-ox, rd22, MYB4, LEA, CHS, and STH-2, and other potential candidates with abundant expression were identified. Our findings provide first insights into gene expression profiles and physiological response for friction combined with GA₃ on A. cantoniensis seed germination.

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摩擦和外源赤霉素对广东草发芽影响的转录组比较分析。

广州草种子在自然条件下萌发率极低，具有休眠特征。一般情况下，外源赤霉素(GA3)可以通过摩擦或浸泡打破其种子休眠。迄今为止，GA3和摩擦对其种子萌发影响的分子机制尚不清楚。在本研究中，我们测试了无菌水浸泡(G1)、摩擦(G2)、GA3浸泡(G3)、摩擦和GA3联合处理(G4)对种子萌发的影响。然后，我们通过RNA测序研究了不同处理对应的种子转录组谱。结果表明，摩擦与GA3联合处理可显著提高种子萌发率。RNA-Seq分析产生84.80 Gb的序列。121776个unigenes中有82996个被注释。对比转录组分析发现，G1组与G2组、G1组与G3组、G1组与G4组分别有1130个、1097个、708个单基因分别表达。此外，还鉴定出了20个与种子萌发相关的候选基因，包括CYP78A5、Bg7s、GA-20-ox、rd22、MYB4、LEA、CHS和STH-2，以及其他表达丰富的潜在候选基因。我们的研究结果首次揭示了摩擦和GA3对广东草种子萌发的基因表达谱和生理反应。

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来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.