OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice.

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Signaling & Behavior Pub Date : 2022-12-31 DOI:10.1080/15592324.2022.2142725

Muhammad Ilyas, Safdar Hussain Shah, Yasunari Fujita, Kyonoshin Maruyama, Kazuo Nakashima, Kazuko Yamaguchi-Shinozaki, Asad Jan

{"title":"OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice.","authors":"Muhammad Ilyas, Safdar Hussain Shah, Yasunari Fujita, Kyonoshin Maruyama, Kazuo Nakashima, Kazuko Yamaguchi-Shinozaki, Asad Jan","doi":"10.1080/15592324.2022.2142725","DOIUrl":null,"url":null,"abstract":"Different abiotic stresses induce OsTZF1, a tandem CCCH-type zinc finger domain gene, in rice. Here, we report that transgenic rice plants overexpressing OsTZF1 under own promoter (POsTZF1:OsTZF1-OX [for overexpression]) transferred to soil showed normal growth similar to vector control plants. The POsTZF1:OsTZF1-OX produced normal leaves without any lesion mimic phenotype and exhibited normal seed setting. The POsTZF1:OsTZF1-OX plants showed significantly increased tolerance to salt and drought stresses and enhanced post stress recovery. Microarray analysis revealed a total of 846 genes up-regulated and 360 genes down-regulated in POsTZF1:OsTZF1-OX salt-treated plants. Microarray analysis of POsTZF1:OsTZF1-OX plants showed the regulation of many abiotic stress tolerance genes. These results suggest that OsTZF1-OX under own promoter show abiotic stress tolerance and produces no pleiotropic effect on phenotype of transgenic rice plant.","PeriodicalId":20232,"journal":{"name":"Plant Signaling & Behavior","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9677997/pdf/","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Signaling & Behavior","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/15592324.2022.2142725","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 2

Abstract

Different abiotic stresses induce OsTZF1, a tandem CCCH-type zinc finger domain gene, in rice. Here, we report that transgenic rice plants overexpressing OsTZF1 under own promoter (P_OsTZF1:OsTZF1-OX [for overexpression]) transferred to soil showed normal growth similar to vector control plants. The P_OsTZF1:OsTZF1-OX produced normal leaves without any lesion mimic phenotype and exhibited normal seed setting. The P_OsTZF1:OsTZF1-OX plants showed significantly increased tolerance to salt and drought stresses and enhanced post stress recovery. Microarray analysis revealed a total of 846 genes up-regulated and 360 genes down-regulated in P_OsTZF1:OsTZF1-OX salt-treated plants. Microarray analysis of P_OsTZF1:OsTZF1-OX plants showed the regulation of many abiotic stress tolerance genes. These results suggest that OsTZF1-OX under own promoter show abiotic stress tolerance and produces no pleiotropic effect on phenotype of transgenic rice plant.

Abstract Image

查看原文本刊更多论文

OsTZF1是一个ccch串联锌指蛋白基因，在自身启动子驱动下不产生多效性，使水稻具有耐盐和耐旱性。

不同的非生物胁迫诱导水稻串联ccch型锌指结构域基因OsTZF1。在此，我们报道了在自身启动子(POsTZF1:OsTZF1- ox[过表达])下过表达OsTZF1的转基因水稻植株转移到土壤中后，表现出与病媒介对照植株相似的正常生长。POsTZF1:OsTZF1-OX产生正常叶片，无任何病变模拟表型，并表现出正常的结实率。POsTZF1:OsTZF1-OX植株对盐胁迫和干旱胁迫的耐受性显著提高，胁迫后恢复能力增强。微阵列分析显示，在盐处理过的植物中，有846个基因上调，360个基因下调。对POsTZF1:OsTZF1-OX植株的微阵列分析显示，该植株可调控多种非生物抗逆性基因。上述结果表明，在自身启动子作用下，OsTZF1-OX表现出非生物抗逆性，对转基因水稻植株的表型不产生多效性影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Signaling & Behavior Agricultural and Biological Sciences-Plant Science

CiteScore

6.00

自引率

3.40%

发文量

111

期刊介绍： Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.