In vitro Anti-hemolytic Effect, in vivo Anti-inflammatory and in vitro Anti-oxidant Activity of Anchusa azurea Mill.

Q2 Medicine

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pub Date : 2022-01-01 DOI:10.2174/1871523020666211201162917

Naouel Boussoualim, Hayat Trabsa, Imane Krache, Soraya Ouhida, Lekhmissi Arrar, Abderrahmane Baghiani

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引用次数: 0

Abstract

Background: Anchusa azurea Mill. (AA) is a medicinal plant largely used traditionally in folk medicine in Algeria; it is locally named hamham. It is effective in the treatment of various diseases.

Objectives: The aim of the present study is to determine the antioxidant, anti-inflammatory, and anti- hemolytic effects of phenolic fractions from Anchusa azurea Mill.

Methods: In this study, various extracts from Anchusa azurea Mill. (AA) using solvents with increasing polarity were prepared. The quantification of polyphenols and flavonoids was determined. The anti-radical activity of the different extracts was evaluated using DPPH and by measuring the inhibition of the oxidative degradation of β-carotene. The In-vitro antihemolytic effect of the plant extracts is determined (CrE, ChE, AcE, and AqE). For each extract, four concentrations were tested: 10.59, 21.18, 42.37, 84.74 μg/ml. Vitamin C is used as a standard. The free-radical attack was measured by measuring the HT₅₀ (Half-Hemolysis Time). The anti-inflammatory effect using PMA on mice of the methanolic extract (CrE) was evaluated.

Results: The quantification of polyphenols and flavonoids showed that ethyl acetate extract (AcE) contains a higher amount of polyphenols. However, chloroform extract (ChE) presents a higher amount of flavonoids. AcE showed an important scavenging activity using the DPPH radical (IC₅₀= 68.35 μg/ml). The results showed that AcE also exhibited a significant inhibition effect on the oxidation of β-carotene/linoleic acid (84.33 %). All extracts increased the HT50 values (Half-Hemolysis Time) in a dose-dependent manner. The three highest concentrations (21.18, 42.37, and 84.74 μg / ml) of ChE caused a very significant delay (p ≤ 0.001) of hemolysis compared to the negative control and the positive control "VIT C". The anti-inflammatory effect of using PMA on mice showed that the methanolic extract (CrE) of AA reduced the weight of the ear edema.

Conclusion: This plant has a strong pharmacological power, which supports its traditional medicinal use.

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蓝齿莲的体外抗溶血作用、体内抗炎和体外抗氧化活性。

背景:Anchusa azurea Mill。(AA)是一种药用植物，主要用于阿尔及利亚的传统民间医药;它在当地被称为hamham。它对治疗各种疾病都有效。目的:研究鹤嘴草酚类成分的抗氧化、抗炎和抗溶血作用。方法:本研究采用不同提取方法，对不同提取物进行提取。(AA)采用极性递增的溶剂制备。测定了黄酮类和多酚类物质的含量。采用DPPH法和测定其对β-胡萝卜素氧化降解的抑制作用来评价不同提取物的抗自由基活性。测定了植物提取物的体外抗溶血作用(CrE、ChE、AcE和AqE)。对每种提取物分别检测4种浓度:10.59、21.18、42.37、84.74 μg/ml。维生素C被用作标准。采用半溶血时间(HT50)测定自由基攻击。评价PMA对小鼠甲醇提取物(CrE)的抗炎作用。结果:乙酸乙酯提取物(AcE)中多酚和黄酮类化合物含量较高。然而，氯仿提取物(ChE)的黄酮类化合物含量较高。AcE对DPPH自由基具有明显的清除作用(IC50= 68.35 μg/ml)。结果表明，AcE对β-胡萝卜素/亚油酸的氧化也有显著的抑制作用(84.33%)。所有提取物均以剂量依赖性方式增加HT50值(半溶血时间)。与阴性对照和阳性对照“VIT C”相比，三个最高浓度(21.18、42.37和84.74 μg / ml)的ChE对溶血的延迟非常显著(p≤0.001)。PMA对小鼠的抗炎作用表明，AA乙醇提取物(CrE)减轻了小鼠耳水肿的重量。结论:该植物具有较强的药理作用，支持其传统药用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

3.30

自引率

0.00%

发文量

期刊介绍： Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new anti-inflammatory & anti-allergy agents. Publishing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.