Phyllanthus emblica L. Regulates BDNF/PI3K Pathway to Modulate Glutathione for Mitoprotection and Neuroprotection in a Rodent Model of Ischemic Stroke.

Q3 Psychology
Deepaneeta Sarmah, Geetesh Verma, Aishika Datta, Namrata Vadak, Antra Chaudhary, Kiran Kalia, Pallab Bhattacharya
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引用次数: 0

Abstract

Introduction: Ischemic stroke remains the leading cause of death worldwide and is the primary cause of disability globally. Numerous studies have shown that plant-origin medicines are promising and can influence the treatment of neurological disorders. Phyllanthus embilica L. (P. emblica or Amla) is one of the herbal plants whose medicinal properties are widely studied. The objective of the present study is to determine the neuroprotective effects of an aqueous extract of the fruit of P. emblica (hereinafter referred to as just P. emblica) on cerebral ischemia-reperfusion injury and explore if it can regulate BDNF/PI3K pathway to modulate glutathione for mitoprotection and neuroprotection.

Methods: In vivo studies were conducted on male Sprague Dawley rats, where rats were prophylactically administered 100 mg/kg P. emblica for 30 days. In the treatment group, rats were given 100 mg/kg P. emblica, 1 h post middle cerebral artery occlusion (MCAo). Rats were evaluated for neuro deficit and motor function tests. Brains were further harvested for infarct size evaluation, biochemical analysis, protein expression studies, and mitochondrial studies.

Results: Prophylaxis and treatment with P. emblica demonstrated significant improvement in functional outcome with a reduction in infarct size. Normalization of glutathione, nitrite, and malondialdehyde levels was also observed. Improvement in mitochondrial complex I and IV activities was also reported. Expressions of BDNF, PI3K, SDF1 and VEGF increased while that of ROCK2 decreased following P. emblica administration.

Conclusion: P. emblica regulates BDNF/PI3K pathway to modulate glutathione in ischemic stroke to confer mitoprotection and neuroprotection.

余甘子调节BDNF/PI3K通路调节谷胱甘肽对缺血性脑卒中鼠模型的有丝分裂保护和神经保护作用
缺血性脑卒中仍然是全球死亡的主要原因,也是全球残疾的主要原因。许多研究表明,植物来源的药物很有前途,可以影响神经系统疾病的治疗。甘油三叶(Phyllanthus embilica L., P. emblica或Amla)是药用价值被广泛研究的草本植物之一。本研究的目的是确定南牛蒡子水提物(以下简称南牛蒡子)对脑缺血-再灌注损伤的神经保护作用,并探讨其是否通过调节BDNF/PI3K通路调节谷胱甘肽起到有丝分裂保护和神经保护的作用。方法:雄性Sprague Dawley大鼠体内实验,大鼠预防性给药100 mg/kg栓皮炎30 d。治疗组大鼠在大脑中动脉闭塞(MCAo) 1 h后给予栓皮炎100 mg/kg。对大鼠进行神经缺损和运动功能测试。进一步采集脑用于梗死面积评估、生化分析、蛋白表达研究和线粒体研究。结果:通过减少梗死面积,预防和治疗emblica可显著改善功能结果。还观察到谷胱甘肽、亚硝酸盐和丙二醛水平的正常化。线粒体复合体I和IV活性也有所改善。大鼠给药后BDNF、PI3K、SDF1、VEGF表达升高,ROCK2表达降低。结论:鹰钩藤通过BDNF/PI3K通路调节谷胱甘肽对缺血性脑卒中有丝分裂保护和神经保护作用。
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来源期刊
Central nervous system agents in medicinal chemistry
Central nervous system agents in medicinal chemistry Psychology-Neuropsychology and Physiological Psychology
CiteScore
2.10
自引率
0.00%
发文量
21
期刊介绍: Central Nervous System Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of new central nervous system agents. Containing a series of timely in-depth reviews written by leaders in the field covering a range of current topics, Central Nervous System Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in the field.
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