Transcriptome Sequencing Analysis of the Effect of β-Elemene on Colorectal Cancer from the lncRNA-miRNA-mRNA Perspective.

IF 1.4 4区生物学 Q4 GENETICS & HEREDITY

Genetics research Pub Date : 2022-01-01 DOI:10.1155/2022/5896296

Heng Deng, Shuo Chen, Xiancang Yuan, Jun Zhang

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引用次数: 2

Abstract

Object. β-Elemene is an emerging antitumor Chinese medicine, but the exact mechanism of action of β-elemene in colorectal cancer (CRC) remains unclear. This study aimed to explore the mechanism of the lncRNA-miRNA-mRNA network in the process of β-elemene inhibiting CRC. Methods. RNA sequencing was performed on CRC cells from the control group (untreated) and the case group (β-elemene-treated). According to the sequencing data, we screened the differentially expressed (DE) lncRNAs, miRNAs, and mRNAs and then analyzed them by functional enrichment analyses. Through the lncRNA-miRNA-mRNA network, the key miRNAs and mRNAs involved in the process of β-elemene inhibiting CRC were further identified. Results. Totally, 607 upregulated and 599 downregulated DElncRNAs, 12 downregulated and 24 upregulated DEmiRNAs, and 3153 downregulated and 3248 upregulated DEmRNAs were identified. Through the lncRNA-miRNA-mRNA network, 3 miRNAs (miR-7109-3p, miR-4506, and miR-3182), 7 prognostic mRNAs (ALPG, DTX1, HOXD13, RIMS3, SLC16A8, SYT1, and TNNT1), and 2 key mRNAs (RIMS3 and SLC16A8) were determined to participate in the inhibitory mechanism of β-elemene in CRC. Conclusion. This study revealed for the first time that the lncRNA-miRNA-mRNA network is involved in the regulation of β-elemene in CRC, and these identified miRNAs and mRNAs could be new clinical prognostic biomarkers and therapeutic targets for CRC patients.

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从lncRNA-miRNA-mRNA的角度分析β-榄香烯对结直肠癌的影响

对象。β-榄香烯是一种新兴的抗肿瘤中药，但其在结直肠癌中的确切作用机制尚不清楚。本研究旨在探讨lncRNA-miRNA-mRNA网络在β-榄香烯抑制CRC过程中的作用机制。方法。对对照组(未处理)和病例组(β-榄香烯处理)的CRC细胞进行RNA测序。根据测序数据，我们筛选了差异表达(DE)的lncrna、mirna和mrna，然后通过功能富集分析对其进行分析。通过lncRNA-miRNA-mRNA网络，进一步鉴定了参与β-榄香烯抑制CRC过程的关键mirna和mrna。结果。总共鉴定出607个delncrna上调，599个下调，12个demirna下调，24个demirna上调，3153个demrna下调，3248个demrna上调。通过lncRNA-miRNA-mRNA网络，我们确定了3种mirna (miR-7109-3p、miR-4506、miR-3182)， 7种预后mrna (ALPG、DTX1、HOXD13、RIMS3、SLC16A8、SYT1、TNNT1)和2种关键mrna (RIMS3、SLC16A8)参与了β-烯在结直肠癌中的抑制机制。结论。本研究首次揭示了lncRNA-miRNA-mRNA网络参与了结直肠癌中β-榄香烯的调控，这些鉴定的mirna和mrna可能成为结直肠癌患者新的临床预后生物标志物和治疗靶点。

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来源期刊

Genetics research 生物-遗传学

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Genetics Research is a key forum for original research on all aspects of human and animal genetics, reporting key findings on genomes, genes, mutations and molecular interactions, extending out to developmental, evolutionary, and population genetics as well as ethical, legal and social aspects. Our aim is to lead to a better understanding of genetic processes in health and disease. The journal focuses on the use of new technologies, such as next generation sequencing together with bioinformatics analysis, to produce increasingly detailed views of how genes function in tissues and how these genes perform, individually or collectively, in normal development and disease aetiology. The journal publishes original work, review articles, short papers, computational studies, and novel methods and techniques in research covering humans and well-established genetic organisms. Key subject areas include medical genetics, genomics, human evolutionary and population genetics, bioinformatics, genetics of complex traits, molecular and developmental genetics, Evo-Devo, quantitative and statistical genetics, behavioural genetics and environmental genetics. The breadth and quality of research make the journal an invaluable resource for medical geneticists, molecular biologists, bioinformaticians and researchers involved in genetic basis of diseases, evolutionary and developmental studies.