The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.

IF 1.1 4区 医学 Q3 SURGERY
Acta cirurgica brasileira Pub Date : 2022-12-12 eCollection Date: 2022-01-01 DOI:10.1590/acb370904
Xuan Yang, Hui Yu, Jingli Wei, Qiuyan Wei, Hui Huang, Jing Chen, Jianzhe Li, Shuyi Yu
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Abstract

Purpose: This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).

Methods: The rats from C. butyricum group were given C. butyricum for 5 days. Then, hepatic ischemia for 30 min and reperfusion for 6 h were performed in all the rats. After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.

Results: The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.

Conclusions: C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress. These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.

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膳食中补充丁酸梭菌对大鼠肝缺血再灌注损伤的保护作用
目的:本研究探讨了口服丁酸梭菌(C. butyricum)对肝缺血再灌注损伤(HIRI)大鼠炎症、氧化应激和肠道菌群的影响:方法:给丁酸组大鼠服用丁酸 5 天。然后,对所有大鼠进行肝缺血 30 分钟和再灌注 6 小时。动物被处死后,检测粪便中的丙氨酸转氨酶(ALT)、天冬氨酸氨基转移酶(AST)、血清中的脂多糖(LPS)、短链脂肪酸(SCFAs)和肠道微生物群组成、以及丙二醛(MDA)、谷胱甘肽(GSH)、肿瘤坏死因子-α(TNF-α)、白细胞介素 6(IL-6)、Toll 样受体 4(TLR4)、核因子-kappa Bp65(NF-κBp65)和肝脏组织学分析。结果服用丁酸杆菌的大鼠的谷丙转氨酶(ALT)、谷草转氨酶(AST)、LPS 和 MDA 均有所下降;谷胱甘肽(GSH)和组织学损伤有所改善;SCFAs 发生变化;TNF-α、IL-6、TLR4 和 pNF-κBp65/NF-κBp65 均有所下降;肠道微生物组成发生变化,其中固着菌/类杆菌比例下降,益生菌相对丰度(RA)增加:结论:补充丁酸杆菌可通过调节肠道微生物组成来预防 HIRI,这有助于降低 LPS 并减轻炎症和氧化应激。这表明丁酸杆菌可能是治疗 HIRI 的有效临床术前膳食补充剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.90
自引率
9.10%
发文量
60
审稿时长
3-8 weeks
期刊介绍: Information not localized
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