Manganese Exposure Induces Cellular Aggregates and the Accumulation of β-Catenin in Skin of Zebrafish Embryos.

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Accounts of Chemical Research Pub Date : 2023-08-01 Epub Date: 2023-07-05 DOI:10.1089/zeb.2022.0056
Geyse Gomes, José Leonardo Oliveira, Manoel Luis Costa, Claudia Mermelstein, Natália Martins Feitosa
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Abstract

The effects of manganese (Mn) toxicity in different organs and tissues in humans and other vertebrates have been studied since the beginning of the past century, but most of its cellular effects remain largely unknown. In this study, we studied the effects of Mn in zebrafish, at the cellular level, due to the transparent nature of zebrafish larvae that enables a powerful analysis under the light microscope. The collection of our results shows that environmental concentrations of 0.5 mg/L affect swim bladder inflation; at concentration of 50 and 100 mg/L Mn (1) induces alterations in viability, swim bladder, heart, and size of zebrafish larvae, (2) induces an increase in melanocyte area and the formation of cellular aggregates in the skin, and (3) induces an accumulation of β-Catenin in mesenchymal cells in the caudal fin of zebrafish larvae. Our data suggest that increased levels of Mn induce cell aggregate formation in the skin and the presence of more melanocytes in the zebrafish caudal fin. Interestingly, the adhesion protein β-Catenin was activated in mesenchymal cells near the cell aggregates. These results open important new questions on the role of Mn toxicity on cellular organization and β-Catenin responses in fishes.

锰暴露诱导斑马鱼胚胎皮肤细胞聚集和β-儿茶素的积累。
自上个世纪初以来,人们一直在研究锰对人类和其他脊椎动物不同器官和组织的毒性作用,但其大多数细胞作用在很大程度上仍然未知。在这项研究中,我们在细胞水平上研究了锰对斑马鱼的影响,因为斑马鱼幼虫的透明性使我们能够在光学显微镜下进行强有力的分析。我们收集的结果表明,环境浓度为0.5 mg/L影响膀胱充气;浓度为50和100 mg/L Mn(1)诱导斑马鱼幼虫的生存能力、膀胱、心脏和大小的改变,(2)诱导皮肤中黑色素细胞面积的增加和细胞聚集体的形成,以及(3)诱导斑马鱼幼虫尾鳍间充质细胞中β-儿茶素的积累。我们的数据表明,Mn水平的增加会诱导皮肤中细胞聚集物的形成,以及斑马鱼尾鳍中更多黑色素细胞的存在。有趣的是,粘附蛋白β-儿茶素在细胞聚集体附近的间充质细胞中被激活。这些结果为锰毒性对鱼类细胞组织和β-儿茶素反应的作用提出了重要的新问题。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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