{"title":"A pathogen imprinted hybrid polymer capacitive sensor for selective Escherichia coli detection","authors":"Samuel M. Mugo, Weihao Lu, Dhanjai Dhanjai","doi":"10.1002/mds3.10071","DOIUrl":null,"url":null,"abstract":"<p>A versatile pathogen imprinted polymer electrochemical sensor has been developed for <i>Escherichia coli</i> detection. The sensor is based on layer by layer assembly of multi-walled carbon nanotubes (CNT), nanocellulose (CNC) films, integrated with polyaniline (PANI) doped phenylboronic acid (PBA). The sensing layer is a poly(methacrylic acid) based pathogen microcontact imprinted polymer (PIP). The PIP@PBA/PANI@CNT/CNC sensor exhibits a high affinity towards <i>E. coli</i> in real biological matrices, while effective in discriminating <i>E. coli</i> amidst other bacteria. Using capacitance and impedance as transduction methods, the PIP sensor recorded a low limit of <i>E. coli</i> detection of 8.7 ± 0.5 cfu/ml, with a rapid response of ≤5 min.</p>","PeriodicalId":87324,"journal":{"name":"Medical devices & sensors","volume":"3 2","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/mds3.10071","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical devices & sensors","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mds3.10071","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 7
Abstract
A versatile pathogen imprinted polymer electrochemical sensor has been developed for Escherichia coli detection. The sensor is based on layer by layer assembly of multi-walled carbon nanotubes (CNT), nanocellulose (CNC) films, integrated with polyaniline (PANI) doped phenylboronic acid (PBA). The sensing layer is a poly(methacrylic acid) based pathogen microcontact imprinted polymer (PIP). The PIP@PBA/PANI@CNT/CNC sensor exhibits a high affinity towards E. coli in real biological matrices, while effective in discriminating E. coli amidst other bacteria. Using capacitance and impedance as transduction methods, the PIP sensor recorded a low limit of E. coli detection of 8.7 ± 0.5 cfu/ml, with a rapid response of ≤5 min.
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