Chlamydia psittaci inhibits apoptosis of human neutrophils by activating P2X7 receptor expression

IF 4.5 3区医学 Q1 MICROBIOLOGY

International Journal of Medical Microbiology Pub Date : 2022-12-01 DOI:10.1016/j.ijmm.2022.151571

Zhangping He , Chuan Wang , Jianye Wang , Kang Zheng , Nan Ding , Maoying Yu , Weiwei Li , Yuanyuan Tang , Yumeng Li , Jian Xiao , Mingxing Liang , Yimou Wu

{"title":"Chlamydia psittaci inhibits apoptosis of human neutrophils by activating P2X7 receptor expression","authors":"Zhangping He , Chuan Wang , Jianye Wang , Kang Zheng , Nan Ding , Maoying Yu , Weiwei Li , Yuanyuan Tang , Yumeng Li , Jian Xiao , Mingxing Liang , Yimou Wu","doi":"10.1016/j.ijmm.2022.151571","DOIUrl":null,"url":null,"abstract":"<div>This study tested the hypothesis that Chlamydia psittaci (C. psittaci) survives and multiplies in human neutrophils by activating P2X7, a nonselective cationic channel receptor expressed constitutively on the surface of these cells. Findings illustrated that P2X7 receptor expression was enhanced in C. psittaci-infected neutrophils. C. psittaci was able to inhibite spontaneous apoptosis of neutrophils through mitochondrial-induced ATP release and IL-8 production. Importantly, inhibiting ATP activation of the P2X7 receptor with AZ10606120 promotes apoptosis, while stimulating P2X7 receptor expression with BzATP delayed spontaneous apoptosis of human neutrophils, suggesting that C. psittaci inhibits apoptosis of human neutrophils by activating P2X7 receptor. This study reveals new insights into the survival advantages of the latent persistent state of C. psittaci and the mechanism by which it evades the innate immune response.</div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"312 8","pages":"Article 151571"},"PeriodicalIF":4.5000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422122000248/pdfft?md5=a7dee146ab183179e7a671e3e1ac4434&pid=1-s2.0-S1438422122000248-main.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Medical Microbiology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1438422122000248","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 1

Abstract

This study tested the hypothesis that Chlamydia psittaci (C. psittaci) survives and multiplies in human neutrophils by activating P2X7, a nonselective cationic channel receptor expressed constitutively on the surface of these cells. Findings illustrated that P2X7 receptor expression was enhanced in C. psittaci-infected neutrophils. C. psittaci was able to inhibite spontaneous apoptosis of neutrophils through mitochondrial-induced ATP release and IL-8 production. Importantly, inhibiting ATP activation of the P2X7 receptor with AZ10606120 promotes apoptosis, while stimulating P2X7 receptor expression with BzATP delayed spontaneous apoptosis of human neutrophils, suggesting that C. psittaci inhibits apoptosis of human neutrophils by activating P2X7 receptor. This study reveals new insights into the survival advantages of the latent persistent state of C. psittaci and the mechanism by which it evades the innate immune response.

查看原文本刊更多论文

鹦鹉热衣原体通过激活P2X7受体表达抑制人中性粒细胞凋亡

本研究验证了鹦鹉热衣原体(C.鹦鹉热衣原体)通过激活P2X7在人类中性粒细胞中存活和繁殖的假设，P2X7是一种非选择性阳离子通道受体，在这些细胞表面组成性地表达。结果表明，P2X7受体在鹦鹉螺感染的中性粒细胞中表达增强。鹦鹉螺能够通过线粒体诱导的ATP释放和IL-8的产生抑制中性粒细胞的自发凋亡。重要的是，AZ10606120抑制P2X7受体的ATP激活可促进细胞凋亡，而BzATP刺激P2X7受体的表达可延缓人中性粒细胞的自发凋亡，提示鹦鹉蜡通过激活P2X7受体抑制人中性粒细胞的凋亡。本研究揭示了鹦鹉螺潜伏持续状态的生存优势及其逃避先天免疫应答的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Medical Microbiology 医学-病毒学

CiteScore

9.70

自引率

0.00%

发文量

审稿时长

45 days

期刊介绍： Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.