Loop-Mediated Isothermal Amplification-Based Microfluidic Platforms for the Detection of Viral Infections.

IF 3.1 4区医学 Q2 INFECTIOUS DISEASES

Current Infectious Disease Reports Pub Date : 2022-01-01 DOI:10.1007/s11908-022-00790-5

Yuanyuan Zeng, Chuan Wu, Yang He

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引用次数: 3

Abstract

Purpose of review: Easy-to-use, fast, and accurate virus detection method is essential for patient management and epidemic surveillance, especially during severe pandemics. Loop-mediated isothermal amplification (LAMP) on a microfluidic platform is suitable for detecting infectious viruses, regardless of the availability of medical resources. The purpose of this review is to introduce LAMP-based microfluidic devices for virus detection, including their detection principles, methods, and application.

Recent findings: Facing the uncontrolled spread of viruses, the large-scale deployment of LAMP-based microfluidic platforms at the grassroots level can help expand the coverage of nucleic acid testing and shorten the time to obtain test reports. Microfluidic chip technology is highly integrated and miniaturized, enabling precise fluid control for effective virus detection. Performing LAMP on miniaturized systems can reduce analysis time, reagent consumption and risk of sample contamination, and improve analytical performance.

Summary: Compared to traditional benchtop protocols, LAMP-based microfluidic devices reduce the testing time, reagent consumption, and the risk of sample contamination. In addition to simultaneous detection of multiple target genes by special channel design, microfluidic chips can also integrate digital LAMP to achieve absolute quantification of target genes.

Abstract Image

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基于环介导等温扩增的病毒感染检测微流控平台。

综述目的:简便、快速、准确的病毒检测方法对于患者管理和疫情监测至关重要，特别是在严重的大流行期间。微流控平台上的环介导等温扩增(LAMP)适用于检测感染性病毒，无论医疗资源是否可用。本文综述了基于lamp的微流控病毒检测装置的检测原理、方法及其应用。近期研究发现:面对病毒不受控制的传播，在基层大规模部署基于lamp的微流控平台，有助于扩大核酸检测覆盖面，缩短获取检测报告的时间。微流控芯片技术高度集成和小型化，能够精确控制流体，有效检测病毒。在小型系统上执行LAMP可以减少分析时间，试剂消耗和样品污染风险，并提高分析性能。总结:与传统的台式方案相比，基于lamp的微流体装置减少了测试时间、试剂消耗和样品污染风险。微流控芯片除了通过特殊通道设计同时检测多个靶基因外，还可以集成数字LAMP，实现靶基因的绝对定量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Infectious Disease Reports INFECTIOUS DISEASES-

CiteScore

6.70

自引率

0.00%

发文量

期刊介绍： This journal intends to provide clear, insightful, balanced contributions by international experts that review the most important, recently published clinical findings related to the diagnosis, treatment, management, and prevention of infectious disease. We accomplish this aim by appointing international authorities to serve as Section Editors in key subject areas, such as HIV/AIDS, sexually transmitted diseases, tropical and travel medicine, and urinary tract infections. Section Editors, in turn, select topics for which leading experts contribute comprehensive review articles that emphasize new developments and recently published papers of major importance, highlighted by annotated reference lists.