Automated complete blood counter, urine analyzer and urine dipstick test results are correlated with thoma cell counting chamber counts in the diagnosis of dialysis related peritonitis in children

IF 0.7 4区医学 Q4 UROLOGY & NEPHROLOGY

Nephrologie & Therapeutique Pub Date : 2022-12-01 DOI:10.1016/j.nephro.2022.10.001

Şükran Keskin Gözmen , Erkin Serdaroğlu , Nida Dinçel , Pınar Erturgut , Cemaliye Başaran , Fatma Devrim , Betül Pehlivan Zorlu , Özlem Dur , Orhan Deniz Kara , Ebru Bekiroğlu Yilmaz

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引用次数: 0

Abstract

Introduction

Peritoneal dialysis is the treatment of choice for end-stage renal disease. Peritoneal dialysis related peritonitis is of great importance for patient and technical survival. The aim of our study was to evaluate the accuracy and the correlation between the three methods (complete blood count, urinalysis device, urine dipstick test) and with the reference manual method (Thoma Cell Counter Chamber).

Materials and methods

We retrospectively analyzed 167 peritoneal fluid samples taken from 25 patients receiving peritoneal dialysis treatment. Leukocyte counts were evaluated with Thoma Cell Counter Chamber, complete blood count, urinalysis device and urine dipstick test.

Results

There was a significant positive correlation between Thoma Cell Counter Chamber and complete blood count results (Spearman's rho = 0.70), between Thoma Cell Counter Chamber and urinalysis device (Spearman's rho = 0.73), and between Thoma Cell Counter Chamber and urine dipstick test (Spearman's rho = 0.71). Area under curve for complete blood count, urinalysis device and urine dipstick test were 0.93, 0.94 and 0.89 respectively, indicating good accuracy. Sensitivity and specificity were 89.7% and 86.7% in the complete blood count analysis (associated criterion: 130 cells/mm³). Sensitivity and specificity were 89.7% and 86.7% in the urinalysis device (associated criterion: 10 cells/HPF). Sensitivity and specificity were 79.6% and 91.4% when in the urine dipstick test analysis (associated criterion: + 1 positivity). The Bland-Altman plot showed good agreement.

Conclusion

Automatic complete blood count and urinalysis devices have good correlation and agreement with manual method in the diagnosis of peritonitis in the pediatric age group. Urine dipstick test in the home setting can be useful for screening patients with suspected peritonitis.

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全自动全血计数器、尿液分析仪和尿试纸试验结果与肿瘤细胞计数室计数在儿童透析相关性腹膜炎诊断中的相关性

腹膜透析是治疗终末期肾脏疾病的首选方法。腹膜透析相关性腹膜炎对患者生存和技术生存具有重要意义。本研究的目的是评估三种方法(全血细胞计数、尿液分析装置、尿试纸试验)与参考手册方法(托马细胞计数室)的准确性和相关性。材料和方法回顾性分析25例接受腹膜透析治疗的患者167份腹膜液样本。白细胞计数采用托马细胞计数室、全血细胞计数、尿液分析仪和尿试纸试验。结果与全血细胞计数(Spearman’s rho = 0.70)、与尿检仪(Spearman’s rho = 0.73)、与尿试纸试验(Spearman’s rho = 0.71)呈显著正相关。全血细胞计数曲线下面积为0.93，尿分析仪曲线下面积为0.94，尿试纸曲线下面积为0.89，准确度较好。全血细胞计数分析的敏感性和特异性分别为89.7%和86.7%(相关标准:130个细胞/mm3)。灵敏度和特异性分别为89.7%和86.7%(相关标准:10个细胞/HPF)。尿试纸分析的敏感性和特异性分别为79.6%和91.4%(相关标准为+ 1阳性)。布兰德和奥特曼的图显示了很好的一致性。结论全自动全血细胞计数及尿液分析仪与手工方法诊断小儿腹膜炎具有良好的相关性和一致性。尿试纸试验在家庭环境中可用于筛选疑似腹膜炎的患者。

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来源期刊

Nephrologie & Therapeutique 医学-泌尿学与肾脏学

CiteScore

0.80

自引率

14.30%

发文量

485

审稿时长

11.9 weeks

期刊介绍： Organe d''expression de la Société de Néphrologie, de la Société Francophone de Dialyse et de la Société de Néphrologie Pédiatrique, Néphrologie et Thérapeutique a pour vocation de publier des textes en français dans le domaine de la Néphrologie, qu''il s''agisse d''actualisation des connaissances, de recommandations de bonne pratique clinique, de publications originales, ou d''informations sur la vie des trois sociétés fondatrices. La variété des thèmes abordés reflète la richesse de la Néphrologie, qu''il s''agisse d''aspects fondamentaux issus de la physiologie, de l''immunologie, de l''anatomo-pathologie, ou de la génétique, ou de sujets de néphrologie clinique, notamment ceux en rapport avec les thérapeutiques néphrologiques, transplantation, hémodialyse et dialyse péritonéale.