Role of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry for Species Identification of Acinetobacter Strains.

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL
Anusha Krishnaraj, Pratima Gupta, Mohit Bhatia, Balram Ji Omar
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Abstract

IntroductionAcinetobacter species has become a leading cause of nosocomial infections in recent years. Objectives  The aim of the study was to establish the usefulness of matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS) for the identification of Acinetobacter species with respect to conventional biochemical methods and MicroScan WalkAway 96 Plus system and to compare the antibiotic susceptibility test results Kirby-Bauer disk diffusion method with MicroScan WalkAway 96 Plus automated identification and antimicrobial susceptibility testing system. Materials and Methods  The study sample comprised 100 clinical isolates of Acinetobacter species. They were all identified using MALDI-TOF MS and compared with other two identification systems. Statistical Analysis  Comparison of categorical variables by Fisher's exact test or Pearson's chi-square test was done. All statistical tools were two tailed, and a significant level p  < 0.05 was used. All statistical tests were performed using SPSS v22.0 (Armonk IBM Corp., New York, United States). Cohen's kappa coefficients were also calculated and used as applicable. Results  MALDI-TOF MS revealed 92 A. baumannii , 2 Acinetobacter nosocomialis , 3 Acinetobacter lwoffii , and 1 each was identified as Acinetobacter junii , Acinetobacter johnsonii , and Acinetobacter tandoii . There was moderate agreement between identification by MicroScan WalkAway and MALDI-TOF, and substantial agreement between conventional biochemical tests and MALDI-TOF. We found that there was a 100% categorical agreement with respect to susceptibility of aminoglycosides (amikacin, gentamicin, tobramycin) and cephalosporins (ceftazidime, cefepime, cefotaxime) between disk diffusion method and MicroScan WalkAway 96 Plus system. Total of 16 errors were observed. Conclusion  Although MALDI-TOF MS could be useful to identify A. baumannii but not other species in the genus, it is a rapid, reliable method and can be routinely used in clinical laboratories.

基质辅助激光解吸电离飞行时间质谱法在不动杆菌菌种鉴定中的作用。
近年来,不动杆菌已成为医院感染的主要原因。目的建立基质辅助激光解吸电离飞行时间(MALDI-TOF)质谱法(MS)与常规生化方法和MicroScan WalkAway 96 Plus系统鉴定不动杆菌的有效性,并比较Kirby-Bauer盘片扩散法与MicroScan WalkAway 96 Plus自动鉴定和药敏试验结果。材料与方法采用临床分离的100株不动杆菌作为研究样本。采用MALDI-TOF质谱进行鉴定,并与其他两种鉴定体系进行比较。统计分析采用Fisher精确检验或Pearson卡方检验对分类变量进行比较。结果MALDI-TOF MS检出鲍曼不动杆菌92株,医院不动杆菌2株,lwoffi不动杆菌3株,朱尼不动杆菌、约氏不动杆菌和檀香不动杆菌各1株。MicroScan WalkAway的鉴定结果与MALDI-TOF有中等程度的一致,而传统生化试验结果与MALDI-TOF的鉴定结果基本一致。我们发现圆盘扩散法和MicroScan WalkAway 96 Plus系统对氨基糖苷类药物(阿米卡星、庆大霉素、妥布霉素)和头孢菌素类药物(头孢他啶、头孢吡肟、头孢噻肟)的敏感性有100%的分类一致。共观察到16个错误。结论MALDI-TOF质谱法虽然能鉴别鲍曼不动杆菌属的其他种类,但对该属的其他种类鉴定无效,是一种快速、可靠的方法,可在临床实验室常规应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
自引率
0.00%
发文量
99
审稿时长
31 weeks
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