Pancreatic cancer orthotopic graft in a murine model.

IF 1.1 4区 医学 Q3 SURGERY
Acta cirurgica brasileira Pub Date : 2023-08-04 eCollection Date: 2023-01-01 DOI:10.1590/acb382823
Milena Muzzolini, Ismahane Belhabib, Victoire Cardot, Annemilaï Tijeras-Raballand, Cindy Neuzillet, Corinne Bousquet, Renato Micelli Lupinacci, Christine Jean
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引用次数: 0

Abstract

Purpose: Pancreatic ductal adenocarcinoma (PDAC) is one of the deadliest cancers with increasing incidence. Even if progress have been made, the five-year overall survival remains lower than 10%. There is a desperate need in therapeutic improvements. In the last two decades, new in-vitro models have been developed and improved, including tridimensional-culture spheroids and organoids. However, animal studies remain mandatory in the upscaling before clinical studies. Orthotopic and syngeneic grafting is a robust model to test a drug efficiency in a tumor and its microenvironment.

Methods: We described a method for orthotopic and syngeneic graft of KRAS mutated, p53 wildtype, 8305 cells in a C57BL/6J mouse model.

Results: With this microsurgical method, 30 mice were grafted, 24 by a junior and six by a senior, resulting in 95,8 and 100% of (partial and total) successful tumoral implantation, respectively. Twenty mice underwent ultrasound follow-up. It was an efficient method for the tumoral growth evaluation. At day 16 after grafting, 85% of the tumors were detectable by ultrasound, and at day 22 all tumors were detected.

Conclusions: The presented method appears to be a robust and reliable method for pre-clinical studies. A junior master student can provide positive results using this technique, which can be improved with training.

小鼠模型中的胰腺癌正位移植。
目的:胰腺导管腺癌(PDAC)是最致命的癌症之一,发病率不断上升。即使取得了进展,五年总生存率仍低于 10%。因此亟需改进治疗方法。在过去二十年中,新的体外模型得到了开发和改进,包括三维培养球和有机体。然而,在临床研究之前,动物研究仍然是升级的必经之路。正位和同种异体移植是在肿瘤及其微环境中测试药物疗效的可靠模型:我们介绍了一种在 C57BL/6J 小鼠模型中进行 KRAS 突变、p53 野生型 8305 细胞的正位和同种异体移植的方法:结果:采用这种显微外科方法移植了 30 只小鼠,其中 24 只由低年级学生完成,6 只由高年级学生完成,分别取得了 95,8% 和 100% 的(部分和全部)肿瘤植入成功率。20 只小鼠接受了超声波随访。这是一种评估肿瘤生长的有效方法。移植后第 16 天,85% 的肿瘤可被超声波检测到,第 22 天,所有肿瘤均被检测到:结论:所介绍的方法似乎是一种用于临床前研究的稳健可靠的方法。结论:该方法对于临床前研究而言是一种稳健可靠的方法,初级硕士生也能使用该技术获得积极的结果,而且经过培训后还能得到改善。
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来源期刊
CiteScore
1.90
自引率
9.10%
发文量
60
审稿时长
3-8 weeks
期刊介绍: Information not localized
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