Composition of Sexual Fluids in Cycas revoluta Ovules During Pollination and Fertilization.

IF 2.8 3区生物学 Q2 PLANT SCIENCES

Botanical Review Pub Date : 2022-01-01 DOI:10.1007/s12229-021-09271-1

Patrick von Aderkas, Stefan Little, Massimo Nepi, Massimo Guarnieri, Madeline Antony, Tokushiro Takaso

{"title":"Composition of Sexual Fluids in Cycas revoluta Ovules During Pollination and Fertilization.","authors":"Patrick von Aderkas, Stefan Little, Massimo Nepi, Massimo Guarnieri, Madeline Antony, Tokushiro Takaso","doi":"10.1007/s12229-021-09271-1","DOIUrl":null,"url":null,"abstract":"The composition of fluids that mediate fertilization in cycads is described for the first time. Using tandem mass spectrometry, proteomes of two stages of fluid production, megagametophyte fluid and archegonial chamber fluid production, are compared in Cycas revoluta. These were compared with the proteome of another sexual fluid produced by ovules, the pollination drop proteins. Cycad ovules produce complex liquids immediately prior fertilization. Compared with the pollination drops that mainly had few proteins in classes involved in defense and carbohydrate modification, megagametophyte fluid and archegonial chamber fluid had larger proteomes with many more protein classes, e.g. proteins involved in programmed cell death. Using high-performance liquid chromatography, megagametophyte fluid and archegonial chamber fluid were shown to have elevated concentrations of smaller molecular weight molecules including glucose, pectin and glutamic acid. Compared to megagametophyte fluid, archegonial chamber fluid had elevated pH as well as higher osmolality.Supplementary information: The online version contains supplementary material available at 10.1007/s12229-021-09271-1.","PeriodicalId":55335,"journal":{"name":"Botanical Review","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726676/pdf/","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Botanical Review","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12229-021-09271-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 3

Abstract

The composition of fluids that mediate fertilization in cycads is described for the first time. Using tandem mass spectrometry, proteomes of two stages of fluid production, megagametophyte fluid and archegonial chamber fluid production, are compared in Cycas revoluta. These were compared with the proteome of another sexual fluid produced by ovules, the pollination drop proteins. Cycad ovules produce complex liquids immediately prior fertilization. Compared with the pollination drops that mainly had few proteins in classes involved in defense and carbohydrate modification, megagametophyte fluid and archegonial chamber fluid had larger proteomes with many more protein classes, e.g. proteins involved in programmed cell death. Using high-performance liquid chromatography, megagametophyte fluid and archegonial chamber fluid were shown to have elevated concentrations of smaller molecular weight molecules including glucose, pectin and glutamic acid. Compared to megagametophyte fluid, archegonial chamber fluid had elevated pH as well as higher osmolality.

Supplementary information: The online version contains supplementary material available at 10.1007/s12229-021-09271-1.

Abstract Image

查看原文本刊更多论文

苏铁授粉和受精过程中性液的组成。

本文首次描述了苏铁植物中介导受精的液体组成。采用串联质谱法比较了苏铁巨附生液和卵母室生液两个产液阶段的蛋白质组学。这些蛋白质组与另一种由胚珠产生的性液——授粉滴蛋白进行了比较。苏铁胚珠在受精前立即产生复杂的液体。与主要含有较少防御类和碳水化合物修饰类蛋白质的授粉液滴相比，巨生植物液和细胞器室液具有更大的蛋白质组，具有更多的蛋白质类，例如涉及程序性细胞死亡的蛋白质。利用高效液相色谱法，发现巨附菌液和细胞器室液中葡萄糖、果胶和谷氨酸等较小分子量分子的浓度升高。与巨附植物培养液相比，卵母室培养液pH值升高，渗透压升高。补充信息:在线版本包含补充资料，可在10.1007/s12229-021-09271-1获取。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Botanical Review 生物-植物科学

CiteScore

8.30

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： For over half a century, The Botanical Review has been a leading international journal noted for its in-depth articles on a broad spectrum of botanical fields. Systematics, phytogeography, cladistics, evolution, physiology, ecology, morphology, paleobotany, and anatomy are but a few of the many subjects that have been covered. The Botanical Review draws together outstanding scientists in the field, synthesizes the current knowledge about a specific subject, and promotes the advancement of botany by indicating the gaps in our knowledge and providing new outlooks on the topic.