SARS-CoV-2 Covid-19 Infection During Pregnancy and Differential DNA Methylation in Human Cord Blood Cells From Term Neonates.

IF 3.2 Q2 GENETICS & HEREDITY
Epigenetics Insights Pub Date : 2023-06-30 eCollection Date: 2023-01-01 DOI:10.1177/25168657231184665
Pedro Urday, Suhita Gayen Nee' Betal, Rochelle Sequeira Gomes, Huda B Al-Kouatly, Kolawole Solarin, Joanna Sy Chan, Dongmei Li, Irfan Rahman, Sankar Addya, Rupsa C Boelig, Zubair H Aghai
{"title":"SARS-CoV-2 Covid-19 Infection During Pregnancy and Differential DNA Methylation in Human Cord Blood Cells From Term Neonates.","authors":"Pedro Urday, Suhita Gayen Nee' Betal, Rochelle Sequeira Gomes, Huda B Al-Kouatly, Kolawole Solarin, Joanna Sy Chan, Dongmei Li, Irfan Rahman, Sankar Addya, Rupsa C Boelig, Zubair H Aghai","doi":"10.1177/25168657231184665","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The global pandemic of coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). About 18.4% of total Covid-19 cases were reported in children. Even though vertical transmission from mother to infant is likely to occur at a low rate, exposure to COVID-19 during fetal life may alter DNA methylation patterns with potential long-term effects.</p><p><strong>Objective: </strong>To determine if COVID-19 infection during pregnancy alters the DNA methylation patterns in umbilical cord blood cells from term infants and to identify potential pathways and genes affected by exposure to COVID-19 infection.</p><p><strong>Methods: </strong>Umbilical cord blood was collected from 8 infants exposed to COVID-19 during pregnancy and 8 control infants with no COVID-19 exposure. Genomic DNA was isolated from umbilical cord blood cells and genome-wide DNA methylation was performed using Illumina Methylation EPIC Array.</p><p><strong>Results: </strong>119 differentially methylated loci were identified at the FDR level of 0.20 (64 hypermethylated loci and 55 hypomethylated loci) in umbilical cord blood cells of COVID-19 exposed neonates compared to the control group. Important canonical pathways identified by Ingenuity Pathway Analysis (IPA) were related to stress response (corticotropin releasing hormone signaling, glucocorticoid receptor signaling, and oxytocin in brain signaling pathway), and cardiovascular disease and development (nitric oxide signaling in the cardiovascular system, apelin cardiomyocyte signaling pathways, factors promoting cardiogenesis, and renin-angiotensin signaling). The genes affected by the differential methylations were associated with cardiac, renal, hepatic, neurological diseases, developmental and immunological disorders.</p><p><strong>Conclusions: </strong>COVID-19 induces differential DNA methylation in umbilical cord blood cells. The differentially methylated genes may contribute to hepatic, renal, cardiac, developmental and immunological disorders in offspring born to mothers with COVID-19 infection during pregnancy, and their developmental regulation.</p>","PeriodicalId":41996,"journal":{"name":"Epigenetics Insights","volume":null,"pages":null},"PeriodicalIF":3.2000,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/db/10.1177_25168657231184665.PMC10328022.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epigenetics Insights","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/25168657231184665","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: The global pandemic of coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). About 18.4% of total Covid-19 cases were reported in children. Even though vertical transmission from mother to infant is likely to occur at a low rate, exposure to COVID-19 during fetal life may alter DNA methylation patterns with potential long-term effects.

Objective: To determine if COVID-19 infection during pregnancy alters the DNA methylation patterns in umbilical cord blood cells from term infants and to identify potential pathways and genes affected by exposure to COVID-19 infection.

Methods: Umbilical cord blood was collected from 8 infants exposed to COVID-19 during pregnancy and 8 control infants with no COVID-19 exposure. Genomic DNA was isolated from umbilical cord blood cells and genome-wide DNA methylation was performed using Illumina Methylation EPIC Array.

Results: 119 differentially methylated loci were identified at the FDR level of 0.20 (64 hypermethylated loci and 55 hypomethylated loci) in umbilical cord blood cells of COVID-19 exposed neonates compared to the control group. Important canonical pathways identified by Ingenuity Pathway Analysis (IPA) were related to stress response (corticotropin releasing hormone signaling, glucocorticoid receptor signaling, and oxytocin in brain signaling pathway), and cardiovascular disease and development (nitric oxide signaling in the cardiovascular system, apelin cardiomyocyte signaling pathways, factors promoting cardiogenesis, and renin-angiotensin signaling). The genes affected by the differential methylations were associated with cardiac, renal, hepatic, neurological diseases, developmental and immunological disorders.

Conclusions: COVID-19 induces differential DNA methylation in umbilical cord blood cells. The differentially methylated genes may contribute to hepatic, renal, cardiac, developmental and immunological disorders in offspring born to mothers with COVID-19 infection during pregnancy, and their developmental regulation.

Abstract Image

Abstract Image

Abstract Image

妊娠期 SARS-CoV-2 Covid-19 感染与足月新生儿脐带血细胞 DNA 甲基化的差异。
背景:2019 年全球冠状病毒疾病大流行(COVID-19)是由严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)引起的。据报告,Covid-19病例总数中约18.4%为儿童。尽管母婴垂直传播的发生率可能很低,但胎儿时期接触 COVID-19 可能会改变 DNA 甲基化模式,从而产生潜在的长期影响:目的:确定孕期感染 COVID-19 是否会改变足月婴儿脐带血细胞的 DNA 甲基化模式,并确定受 COVID-19 感染影响的潜在途径和基因:方法:收集了8名孕期暴露于COVID-19的婴儿和8名未暴露于COVID-19的对照组婴儿的脐带血。从脐带血细胞中分离出基因组 DNA,使用 Illumina Methylation EPIC Array 进行全基因组 DNA 甲基化分析:结果:与对照组相比,在COVID-19暴露的新生儿脐血细胞中发现了119个不同的甲基化位点(64个高甲基化位点和55个低甲基化位点),FDR水平为0.20。通过工程通路分析(IPA)确定的重要典型通路与应激反应(促肾上腺皮质激素释放激素信号通路、糖皮质激素受体信号通路和脑内催产素信号通路)以及心血管疾病和发育(心血管系统中的一氧化氮信号通路、凋亡素心肌细胞信号通路、促进心脏生成的因子和肾素-血管紧张素信号通路)有关。受差异甲基化影响的基因与心脏、肾脏、肝脏、神经系统疾病、发育和免疫疾病有关:结论:COVID-19 能诱导脐带血细胞中不同的 DNA 甲基化。结论:COVID-19会诱导脐带血细胞中DNA甲基化的差异,不同的甲基化基因可能会导致孕期感染COVID-19的母亲所生的后代出现肝脏、肾脏、心脏、发育和免疫疾病,并对其发育产生调节作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Epigenetics Insights
Epigenetics Insights GENETICS & HEREDITY-
CiteScore
5.10
自引率
0.00%
发文量
10
审稿时长
8 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信