Yeast surface display-based identification of ACE2 mutations that modulate SARS-CoV-2 spike binding across multiple mammalian species.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Pete Heinzelman, Jonathan C Greenhalgh, Philip A Romero
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Abstract

Understanding how severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interacts with different mammalian angiotensin-converting enzyme II (ACE2) cell entry receptors elucidates determinants of virus transmission and facilitates development of vaccines for humans and animals. Yeast display-based directed evolution identified conserved ACE2 mutations that increase spike binding across multiple species. Gln42Leu increased ACE2-spike binding for human and four of four other mammalian ACE2s; Leu79Ile had an effect for human and three of three mammalian ACE2s. These residues are highly represented, 83% for Gln42 and 56% for Leu79, among mammalian ACE2s. The above findings can be important in protecting humans and animals from existing and future SARS-CoV-2 variants.

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基于酵母表面展示的 ACE2 突变鉴定可调节多种哺乳动物的 SARS-CoV-2 穗状结合。
了解严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)如何与不同哺乳动物的血管紧张素转换酶 II(ACE2)细胞入口受体相互作用,有助于阐明病毒传播的决定因素,并促进人类和动物疫苗的开发。基于酵母展示的定向进化发现了可增加多个物种尖峰结合的 ACE2 保守突变。Gln42Leu 增加了人类和其他四种哺乳动物 ACE2 中四种的 ACE2 穗状结合;Leu79Ile 对人类和三种哺乳动物 ACE2 中三种有影响。这些残基在哺乳动物 ACE2 中的比例很高,Gln42 和 Leu79 分别占 83% 和 56%。上述发现对于保护人类和动物免受现有和未来的 SARS-CoV-2 变体的感染非常重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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