Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella

IF 4.1 2区医学 Q1 PARASITOLOGY

International Journal for Parasitology: Drugs and Drug Resistance Pub Date : 2023-08-01 DOI:10.1016/j.ijpddr.2023.05.002

Hongtao Zhang , Lei Zhang , Ganglin Ren , Hongbin Si , Xingju Song , Xianyong Liu , Xun Suo , Dandan Hu

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引用次数: 1

Abstract

Worldwide distributed coccidiosis is caused by infection of both Eimeria species and Cystoisospora in the host intestine and causes huge economic losses to the livestock industry, especially the poultry industry. The control of such diseases relies mainly on chemoprophylaxis with anticoccidials, which has led to a very common drug resistance in this field. However, the genetic mechanisms underlying resistance to many anticoccidial drugs remain unknown. In this study, strains of E. tenella resistant to 250 mg/kg monensin were generated and characterized. Forward genetic approaches based on pooled genome sequencing, including experimental evolution and linkage group selection, were used to locate candidate targets responsible for resistance to monensin and diclazuril in E. tenella. A total of 16 nonsynonymous mutants in protein-coding genes were identified in monensin-resistant strains, and two genomic regions with strong selection signals were also detected in diclazuril-resistant strains. Our study reveals the genetic characterization of the experimental evolution and linkage group selection in Eimeria species, and also provides important information that contributes to the understanding of the molecular mechanism of drug resistance in coccidia.

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柔嫩艾美耳球虫耐莫能菌素和双唑齐的正向遗传分析

世界范围内分布的球虫病是由宿主肠道中的艾美耳球虫和囊孢子虫感染引起的，并给畜牧业，特别是家禽业造成巨大的经济损失。这类疾病的控制主要依赖于抗球虫的化学预防，这导致了该领域非常常见的耐药性。然而，对许多抗球虫药物产生耐药性的遗传机制仍然未知。在本研究中，产生并鉴定了对250mg/kg莫能菌素具有抗性的E.tenella菌株。基于合并基因组测序的前瞻性遗传方法，包括实验进化和连锁群选择，被用于定位E.tenella对莫能菌素和双唑脲耐药的候选靶点。在莫能菌素抗性菌株中共鉴定出16个蛋白质编码基因的非同义突变体，在双氯脲抗性菌株中也检测到两个具有强选择信号的基因组区域。我们的研究揭示了艾美耳球虫实验进化和连锁群选择的遗传特征，也为理解球虫耐药性的分子机制提供了重要信息。

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来源期刊

International Journal for Parasitology: Drugs and Drug Resistance PARASITOLOGY-PHARMACOLOGY & PHARMACY

CiteScore

7.90

自引率

7.50%

发文量

审稿时长

48 days

期刊介绍： The International Journal for Parasitology – Drugs and Drug Resistance is one of a series of specialist, open access journals launched by the International Journal for Parasitology. It publishes the results of original research in the area of anti-parasite drug identification, development and evaluation, and parasite drug resistance. The journal also covers research into natural products as anti-parasitic agents, and bioactive parasite products. Studies can be aimed at unicellular or multicellular parasites of human or veterinary importance.