Comparison of Rapid Antigen Detection Test with Reverse Transcription Polymerase Chain Reaction in Highly Suspected COVID-19 Patients.

IF 0.7 4区医学 Q3 MEDICINE, GENERAL & INTERNAL

Jcpsp-Journal of the College of Physicians and Surgeons Pakistan Pub Date : 2023-09-01 DOI:10.29271/jcpsp.2023.09.1058

Muhammad Abdur Rahman Afridi, Zafar Ali, Naveed Iqbal

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引用次数: 0

Abstract

Objective: To compare Rapid Antigen Test (RAT) with Reverse Transcription Polymerase Chain Reaction (RT-PCR) in highly suspected COVID-19 patients and to determine its diagnostic parameters.

Study design: Hospital-based, descriptive/observational study. Place and Duration of the Study: Department of Medicine/COVID Complex, Medical Teaching Institution/Lady Reading Hospital, Peshawar, Khyber Pakhtunkhwa, Pakistan, from October 2021 to April 2022.

Methodology: A total of 300 highly suspected cases of COVID-19 of either gender admitted in the COVID Complex of the hospital, were included. Data from the patients, including RAT and RT-PCR for COVID-19, were collected retrospectively. RT-PCR was used as the reference test and compared with RAT. Diagnostic statistics of RAT, with their respective 95% confidence intervals were calculated for RAT in diagnosing COVID-19, with significance at p ≤0.05.

Results: Among the 300 patients, 137 (45.7%) were males and 163 (54.3%) were females. The mean age was 56.80 ± 13.72 years. On screening, 138 (46%) patients tested positive and 162 (54%) were negative by RAT; whereas 213 (71%) tested positive and 87 (29%) were negative on RT-PCR. The sensitivity and specificity of RAT were 54.5% (95% CI: 47.52%-61.28%) and 74.7% (95% CI: 64.25%-83.42%), respectively. Positive predicted value was 84.1% (95% CI: 78.26%-88.53%) and negative predictive value was 40.1% (95% CI: 35.63%-44.79%). The positive likelihood ratio was 2.15 (95% CI: 1.47-3.15). The negative likelihood-ratio was 0.61(95% CI: 0.50-0.74). The overall accuracy was 60.33% (95% CI: 54.55%-65.91%).

Conclusion: There was a low sensitivity and specificity of the RAT for COVID-19, with an overall accuracy of 60.33%, compared with RT-PCR.

Key words: COVID-19, Rapid Antigen Test, Sensitivity, Specificity, RT-PCR.

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快速抗原检测与逆转录聚合酶链反应在COVID-19高度疑似患者中的比较

目的:比较快速抗原试验(RAT)与逆转录聚合酶链反应(RT-PCR)在COVID-19高度疑似患者中的诊断价值，并探讨其诊断参数。研究设计:基于医院的描述性/观察性研究。研究地点和时间:2021年10月至2022年4月，巴基斯坦开伯尔-普赫图赫瓦省白沙瓦医学教学机构/雷丁夫人医院医学系/COVID综合大楼。方法:纳入该医院新冠肺炎综合医院收治的COVID-19高度疑似病例300例，男女不限。回顾性收集患者的数据，包括COVID-19的RAT和RT-PCR。以RT-PCR作为参考检验，并与RAT进行比较。计算RAT诊断COVID-19的诊断统计，并计算其各自的95%置信区间，p≤0.05为显著性。结果:300例患者中，男性137例(45.7%)，女性163例(54.3%)。平均年龄56.80±13.72岁。在筛查中，138例(46%)患者检测呈阳性，162例(54%)患者检测呈阴性;而RT-PCR阳性213例(71%)，阴性87例(29%)。RAT的敏感性为54.5% (95% CI: 47.52% ~ 61.28%)，特异性为74.7% (95% CI: 64.25% ~ 83.42%)。阳性预测值为84.1% (95% CI: 78.26% ~ 88.53%)，阴性预测值为40.1% (95% CI: 35.63% ~ 44.79%)。阳性似然比为2.15 (95% CI: 1.47 ~ 3.15)。负似然比为0.61(95% CI: 0.50-0.74)。总体准确率为60.33% (95% CI: 54.55% ~ 65.91%)。结论:与RT-PCR相比，RAT对COVID-19的敏感性和特异性较低，总体准确率为60.33%。关键词:COVID-19，快速抗原检测，敏感性，特异性，RT-PCR

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来源期刊

Jcpsp-Journal of the College of Physicians and Surgeons Pakistan MEDICINE, GENERAL & INTERNAL-

CiteScore

1.40

自引率

0.00%

发文量

453

审稿时长

3-6 weeks

期刊介绍： Journal of College of Physicians and Surgeons Pakistan (JCPSP), is the prestigious, peer reviewed monthly biomedical journal of the country published regularly since 1991. Established with the primary aim of promotion and dissemination of medical research and contributed by scholars of biomedical sciences from Pakistan and abroad, it carries original research papers, , case reports, review articles, articles on medical education, commentaries, short communication, new technology, editorials and letters to the editor. It covers the core biomedical health science subjects, basic medical sciences and emerging community problems, prepared in accordance with the “Uniform requirements for submission to bio-medical journals” laid down by International Committee of Medical Journals Editors (ICMJE). All publications of JCPSP are peer reviewed by subject specialists from Pakistan and locally and abroad.