The role of human umbilical cord mesenchymal stem cells-derived exosomal microRNA-431-5p in survival and prognosis of colorectal cancer patients.

IF 2.5 4区医学 Q3 GENETICS & HEREDITY

Mutagenesis Pub Date : 2022-05-04 DOI:10.1093/mutage/geac007

Muwen Qu, Junyi Li, Zifu Hong, Fei Jia, Yinghua He, Lingling Yuan

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引用次数: 8

Abstract

We aim to discuss the role of miR-431-5p in colorectal cancer (CRC) progression via regulating peroxiredoxin 1 (PRDX1). miR-431-5p and PRDX1 expression were detected in CRC tissues and cells, and the relationship between miR-431-5p expression and prognosis of CRC patients was analyzed. Exosomes were extracted from human umbilical cord mesenchymal stem cells (hUCMSCs) and co-cultured with LoVo cells. MTT assay, flow cytometry and Transwell assay were implemented to test cell viability, apoptosis and invasion and migration ability, respectively. The tumor growth was determined as well, and the binding relation between miR-431-5p and PRDX1 was confirmed. miR-431-5p was downregulated and PRDX1 was upregulated in CRC, and miR-431-5p downregulation was associated with poor prognosis. hUCMSC-Exos suppressed the malignant behaviors of LoVo cells, and overexpression of miR-431-5p further aggravated the inhibitory effect of hUCMSC-Exos on LoVo cells. hUCMSC-Exos inhibited PRDX1 expression via miR-431-5p. PRDX1 was targeted by miR-431-5p. miR-431-5p serves as a prognostic biomarker in CRC, and hUCMSC-Exos transfer of miR-431-5p decelerates CRC cell growth by inhibiting PRDX1.

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人脐带间充质干细胞来源的外泌体microRNA-431-5p在结直肠癌患者生存和预后中的作用

我们的目的是讨论miR-431-5p通过调节过氧化物还氧蛋白1 (PRDX1)在结直肠癌(CRC)进展中的作用。在结直肠癌组织和细胞中检测miR-431-5p和PRDX1的表达，分析miR-431-5p表达与结直肠癌患者预后的关系。从人脐带间充质干细胞(hUCMSCs)中提取外泌体并与LoVo细胞共培养。采用MTT法、流式细胞术和Transwell法分别检测细胞活力、细胞凋亡和侵袭迁移能力。同时测定肿瘤生长情况，证实miR-431-5p与PRDX1的结合关系。在结直肠癌中miR-431-5p下调，PRDX1上调，miR-431-5p下调与预后不良相关。hUCMSC-Exos抑制LoVo细胞的恶性行为，过表达miR-431-5p进一步加重了hUCMSC-Exos对LoVo细胞的抑制作用。hUCMSC-Exos通过miR-431-5p抑制PRDX1的表达。PRDX1被miR-431-5p靶向。miR-431-5p作为CRC的预后生物标志物，并且miR-431-5p的hucscs - exos转移通过抑制PRDX1来减缓CRC细胞的生长。

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来源期刊

Mutagenesis 生物-毒理学

CiteScore

5.90

自引率

3.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Mutagenesis is an international multi-disciplinary journal designed to bring together research aimed at the identification, characterization and elucidation of the mechanisms of action of physical, chemical and biological agents capable of producing genetic change in living organisms and the study of the consequences of such changes.