Cell-type specific anti-cancerous effects of nitro-oleic acid and its combination with gamma irradiation.

IF 2.9 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biological Chemistry Pub Date : 2023-09-15 Print Date: 2024-03-25 DOI:10.1515/hsz-2023-0150

Tomas Perecko, Jana Pereckova, Zuzana Hoferova, Martin Falk

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引用次数: 0

Abstract

Nitro-fatty acids (NFAs) are endogenous lipid mediators capable of post-translational modifications of selected regulatory proteins. Here, we investigated the anti-cancerous effects of nitro-oleic acid (NO₂OA) and its combination with gamma irradiation on different cancer cell lines. The effects of NO₂OA on cell death, cell cycle distribution, or expression of p21 and cyclin D1 proteins were analyzed in cancer (A-549, HT-29 and FaDu) or normal cell lines (HGF, HFF-1). Dose enhancement ratio at 50 % survival fraction (DER_IC50) was calculated for samples pre-treated with NO₂OA followed by gamma irradiation. NO₂OA suppressed viability and induced apoptotic cell death. These effects were cell line specific but not in general selective for cancer cells. HT-29 cell line exerted higher sensitivity toward NO₂OA treatment among cancer cell lines tested: induction of cell cycle arrest in the G2/M phase was associated with an increase in p21 and a decrease in cyclin D1 expression. Pre-treatment of HT-29 cells with NO₂OA prior irradiation showed a significantly increased DER_IC50, demonstrating radiosensitizing effects. In conclusion, NO₂OA exhibited potential for combined chemoradiotherapy. Our results encourage the development of new NFAs with improved features for cancer chemoradiation.

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硝基油酸及其与伽马射线照射结合的细胞特异性抗癌作用。

硝基脂肪酸（NFAs）是一种内源性脂质介质，能够对特定的调节蛋白进行翻译后修饰。在此，我们研究了硝基油酸（N2OA）及其与伽马射线照射相结合对不同癌细胞株的抗癌作用。我们分析了硝基油酸对癌症细胞株（A-549、HT-29 和 FaDu）或正常细胞株（HGF、HFF-1）的细胞死亡、细胞周期分布或 p21 和细胞周期蛋白 D1 表达的影响。计算了经 NO2OA 预处理后再进行伽马射线照射的样本在 50% 存活率下的剂量增强比（DERIC50）。NO2OA 可抑制细胞存活率并诱导细胞凋亡。这些作用具有细胞系特异性，但对癌细胞没有普遍的选择性。在测试的癌细胞系中，HT-29 细胞系对 NO2OA 处理具有更高的敏感性：诱导细胞周期停滞在 G2/M 阶段与 p21 的增加和细胞周期蛋白 D1 表达的减少有关。在辐照前用 NO2OA 预处理 HT-29 细胞，可显著提高 DERIC50，显示出放射增敏作用。总之，N2OA 具有联合化放疗的潜力。我们的研究结果鼓励人们开发具有更好特性的新型 NFAs，用于癌症化放疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biological Chemistry 生物-生化与分子生物学

CiteScore

7.20

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Biological Chemistry keeps you up-to-date with all new developments in the molecular life sciences. In addition to original research reports, authoritative reviews written by leading researchers in the field keep you informed about the latest advances in the molecular life sciences. Rapid, yet rigorous reviewing ensures fast access to recent research results of exceptional significance in the biological sciences. Papers are published in a "Just Accepted" format within approx.72 hours of acceptance.