{"title":"<ArticleTitle xmlns:ns0=\"http://www.w3.org/1998/Math/MathML\">Simulation of biochemical dynamics of <ns0:math>\n <ns0:mi>C</ns0:mi>\n <ns0:msup>\n <ns0:mrow class=\"MJX-TeXAtom-ORD\">\n <ns0:mi>a</ns0:mi>\n </ns0:mrow>\n <ns0:mrow class=\"MJX-TeXAtom-ORD\">\n <ns0:mn>2</ns0:mn>\n <ns0:mo>+</ns0:mo>\n </ns0:mrow>\n </ns0:msup>\n</ns0:math> and <ns0:math>\n <ns0:mi>P</ns0:mi>\n <ns0:mi>L</ns0:mi>\n <ns0:mi>C</ns0:mi>\n</ns0:math> in fibroblast cell.","authors":"Ankit Kothiya, Neeru Adlakha","doi":"10.1007/s10863-023-09976-5","DOIUrl":null,"url":null,"abstract":"<p><p>Calcium dynamics is not only responsible for maintaining the framework and functions of the cell but also plays a role in the dynamics of other biochemical systems in the cell. Phospholipase C-<math>\n <mi>γ</mi>\n</math> l (<math>\n <mi>P</mi>\n <mi>L</mi>\n <mi>C</mi>\n</math>) has a crucial role in the function of fibroblast cells. Experiments have shown that <math>\n <mi>P</mi>\n <mi>L</mi>\n <mi>C</mi>\n</math> and <math>\n <mi>C</mi>\n <msup>\n <mrow>\n <mi>a</mi>\n </mrow>\n <mrow>\n <mn>2</mn>\n <mo>+</mo>\n </mrow>\n </msup>\n</math> have interdependent dynamics in fibroblast cells. However, no reaction-diffusion model exists for the two-way feedback system dynamics of <math>\n <mi>C</mi>\n <msup>\n <mrow>\n <mi>a</mi>\n </mrow>\n <mrow>\n <mn>2</mn>\n <mo>+</mo>\n </mrow>\n </msup>\n</math> and <math>\n <mi>P</mi>\n <mi>L</mi>\n <mi>C</mi>\n</math> in fibroblasts till date. The computational model is designed to investigate the impact of variations in several processes, such as the <math>\n <mi>S</mi>\n <mi>E</mi>\n <mi>R</mi>\n <mi>C</mi>\n <mi>A</mi>\n</math> pump, buffer process, source inflow, etc., on the system dynamics of <math>\n <mi>C</mi>\n <msup>\n <mrow>\n <mi>a</mi>\n </mrow>\n <mrow>\n <mn>2</mn>\n <mo>+</mo>\n </mrow>\n </msup>\n</math> and <math>\n <mi>P</mi>\n <mi>L</mi>\n <mi>C</mi>\n</math> in fibroblast cells. The computational findings are obtained using finite element techniques, and the consequences of dysregulation in various processes on the spatiotemporal calcium and <math>\n <mi>P</mi>\n <mi>L</mi>\n <mi>C</mi>\n</math> dynamics in fibroblasts are investigated. The results lead to the conclusion that the effects of buffer, source influx, diffusion, and <math>\n <mi>S</mi>\n <mi>E</mi>\n <mi>R</mi>\n <mi>C</mi>\n <mi>A</mi>\n</math> pump can cause fluctuations in the dynamics of <math>\n <mi>C</mi>\n <msup>\n <mrow>\n <mi>a</mi>\n </mrow>\n <mrow>\n <mn>2</mn>\n <mo>+</mo>\n </mrow>\n </msup>\n</math> and <math>\n <mi>P</mi>\n <mi>L</mi>\n <mi>C</mi>\n</math> in fibroblasts. Disruptions in these constitutive processes can result in changes in the dynamics of calcium and <math>\n <mi>P</mi>\n <mi>L</mi>\n <mi>C</mi>\n</math>. Thus, the current model provides new/novel information regarding the precise dysregulatory constitutive systems that regulate calcium and <math>\n <mi>P</mi>\n <mi>L</mi>\n <mi>C</mi>\n</math> kinetics, such as source inflow, diffusion, <math>\n <mi>S</mi>\n <mi>E</mi>\n <mi>R</mi>\n <mi>C</mi>\n <mi>A</mi>\n</math>, and buffer, can be responsible for excessive calcium and <math>\n <mi>P</mi>\n <mi>L</mi>\n <mi>C</mi>\n</math> concentrations leading to fibrotic illnesses such as cancer and fibrosis.</p>","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":"55 4","pages":"267-287"},"PeriodicalIF":2.9000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Bioenergetics and Biomembranes","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10863-023-09976-5","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/7/26 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0
Abstract
Calcium dynamics is not only responsible for maintaining the framework and functions of the cell but also plays a role in the dynamics of other biochemical systems in the cell. Phospholipase C- l () has a crucial role in the function of fibroblast cells. Experiments have shown that and have interdependent dynamics in fibroblast cells. However, no reaction-diffusion model exists for the two-way feedback system dynamics of and in fibroblasts till date. The computational model is designed to investigate the impact of variations in several processes, such as the pump, buffer process, source inflow, etc., on the system dynamics of and in fibroblast cells. The computational findings are obtained using finite element techniques, and the consequences of dysregulation in various processes on the spatiotemporal calcium and dynamics in fibroblasts are investigated. The results lead to the conclusion that the effects of buffer, source influx, diffusion, and pump can cause fluctuations in the dynamics of and in fibroblasts. Disruptions in these constitutive processes can result in changes in the dynamics of calcium and . Thus, the current model provides new/novel information regarding the precise dysregulatory constitutive systems that regulate calcium and kinetics, such as source inflow, diffusion, , and buffer, can be responsible for excessive calcium and concentrations leading to fibrotic illnesses such as cancer and fibrosis.
期刊介绍:
The Journal of Bioenergetics and Biomembranes is an international journal devoted to the publication of original research that contributes to fundamental knowledge in the areas of bioenergetics, biomembranes, and transport, including oxidative phosphorylation, photosynthesis, muscle contraction, as well as cellular and systemic metabolism. The timely research in this international journal benefits biophysicists, membrane biologists, cell biologists, biochemists, molecular biologists, physiologists, endocrinologists, and bio-organic chemists.