Simulation of biochemical dynamics of C a 2 + and P L C in fibroblast cell.

Abstract

Calcium dynamics is not only responsible for maintaining the framework and functions of the cell but also plays a role in the dynamics of other biochemical systems in the cell. Phospholipase C-$\gamma$ l ($PLC$) has a crucial role in the function of fibroblast cells. Experiments have shown that $PLC$ and $C{a}^{2+}$ have interdependent dynamics in fibroblast cells. However, no reaction-diffusion model exists for the two-way feedback system dynamics of $C{a}^{2+}$ and $PLC$ in fibroblasts till date. The computational model is designed to investigate the impact of variations in several processes, such as the $SERCA$ pump, buffer process, source inflow, etc., on the system dynamics of $C{a}^{2+}$ and $PLC$ in fibroblast cells. The computational findings are obtained using finite element techniques, and the consequences of dysregulation in various processes on the spatiotemporal calcium and $PLC$ dynamics in fibroblasts are investigated. The results lead to the conclusion that the effects of buffer, source influx, diffusion, and $SERCA$ pump can cause fluctuations in the dynamics of $C{a}^{2+}$ and $PLC$ in fibroblasts. Disruptions in these constitutive processes can result in changes in the dynamics of calcium and $PLC$. Thus, the current model provides new/novel information regarding the precise dysregulatory constitutive systems that regulate calcium and $PLC$ kinetics, such as source inflow, diffusion, $SERCA$, and buffer, can be responsible for excessive calcium and $PLC$ concentrations leading to fibrotic illnesses such as cancer and fibrosis.