Evaluation of Bach1 mRNA expression in patients with chronic kidney disease: A preliminary study

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-04-03 DOI:10.1111/hdi.13084

Denise Mafra, Livia Alvarenga, Marcia Ribeiro, Beatriz G. Baptista, Susane Fanton, Bruna R. Paiva, Liana Trugilho, Julie Ann Kemp, Marcelo Alves-Ribeiro, Ludmila F. M. F. Cardozo

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引用次数: 1

Abstract

Introduction

BTB and CNC homology 1 (Bach1) is a protein that antagonizes some actions of nuclear factor erythroid 2-related factor-2 (Nrf2), the master regulator of cytoprotective responses. Bach1 binds to genomic DNA and inhibits the synthesis of antioxidant enzymes, thereby increasing inflammation. Bach1 may be a therapeutic target for mitigating inflammation in chronic kidney disease (CKD) patients. However, no clinical study has been reported on Bach1 in this population. This study aimed to evaluate Bach1 mRNA expression with different treatments for CKD, including conservative treatment (nondialysis), hemodialysis (HD), and peritoneal dialysis (PD).

Methods

Twenty patients undergoing HD (56.5 [19] years), 15 on PD (54 [24] years) and 13 nondialysis patients (63 [10] years, with an estimated glomerular filtration rate of 41 [14] mL/min/1.73 m²) were enrolled in the study. The mRNA expression of Nrf2, NF-kB, heme oxygenase 1 (HO-1), and Bach1 was evaluated in peripheral blood mononuclear cells using quantitative real-time polymerase chain reaction. Malondialdehyde (MDA) was evaluated as a lipid peroxidation marker. Routine biochemical parameters were also evaluated.

Findings

As expected, patients on dialysis were more inflamed. Bach1 mRNA expression was significantly higher in patients undergoing HD than in PD and nondialysis patients (p < 0.007). The mRNA expression of HO-1, NF-kB, and Nrf2 was not different in the groups.

Conclusion

In conclusion, CKD patients on HD exhibited an upregulation of Bach1 mRNA expression compared to patients on PD treatment and nondialysis CKD patients. The association between Nrf2 and Bach1 expression in these patients warrants further investigation.

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慢性肾脏疾病患者Bach1 mRNA表达的初步研究

BTB和CNC同源1 (Bach1)是一种拮抗核因子-红细胞2相关因子-2 (Nrf2)某些作用的蛋白，Nrf2是细胞保护反应的主要调节因子。Bach1与基因组DNA结合，抑制抗氧化酶的合成，从而增加炎症。Bach1可能是减轻慢性肾脏疾病(CKD)患者炎症的治疗靶点。然而，在这一人群中没有关于Bach1的临床研究报道。本研究旨在评估CKD不同治疗方法中Bach1 mRNA的表达，包括保守治疗(非透析)、血液透析(HD)和腹膜透析(PD)。方法入选20例HD患者(56.5[19]年)，15例PD患者(54[24]年)和13例非透析患者(63[10]年，肾小球滤过率估计为41 [14]mL/min/1.73 m2)。采用实时定量聚合酶链反应检测外周血单个核细胞Nrf2、NF-kB、血红素加氧酶1 (HO-1)和Bach1 mRNA的表达。丙二醛(MDA)被评价为脂质过氧化标志物。同时对常规生化指标进行评价。正如预期的那样，透析患者的炎症更严重。HD患者的Bach1 mRNA表达明显高于PD和非透析患者(p < 0.007)。各组HO-1、NF-kB、Nrf2 mRNA表达量无显著差异。综上所述，与PD治疗的患者和非透析的CKD患者相比，HD的CKD患者表现出Bach1 mRNA表达上调。这些患者中Nrf2和Bach1表达之间的关系值得进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.