Enhancement of capsular hypermucoviscosity in Klebsiella pneumoniae by Acanthamoeba.

IF 3.8 2区医学 Q1 Medicine

PLoS Neglected Tropical Diseases Pub Date : 2023-08-11 eCollection Date: 2023-08-01 DOI:10.1371/journal.pntd.0011541

Jian-Ming Huang, Ko-Chiang Sung, Wei-Chen Lin, Hong-Yue Lai, Yu-Jen Wang

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Abstract

Background: Acanthamoeba and Klebsiella pneumoniae are both environmental commensals. Recently, clinical harm caused by hypermucoviscous K. pneumoniae has been observed. However, the interaction between these microbes and the origin of hypermucoviscous K. pneumoniae have not been reported.

Methodology/principal findings: Here, we report that the bacterial capsule is enlarged when co-cultured with Acanthamoeba using India ink staining, and this effect depends on the number of parasites present. This interaction results in an enhancement of capsular polysaccharide production in the subsequent generations of K. pneumoniae, even without co-culturing with Acanthamoeba. The hypermucoviscosity of the capsule was examined using the sedimentation assay and string test. We also screened other K. pneumoniae serotypes, including K1, K2, K5, and K20, for interaction with Acanthamoeba using India ink staining, and found the same interaction effect.

Conclusions/significance: These findings suggest that the interaction between Acanthamoeba and K. pneumoniae could lead to harmful consequences in public health and nosocomial disease control, particularly hypermucoviscous K. pneumoniae infections.

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棘阿米巴对肺炎克雷伯菌荚膜高粘附度的增强作用。

背景：棘阿米巴和肺炎克雷伯菌均为环境共生菌。近年来，人们观察到高粘肺炎克雷伯菌引起的临床危害。然而，这些微生物与高粘肺炎克雷伯菌起源之间的相互作用尚未报道。方法/主要发现：在这里，我们报告了使用印度墨水染色与棘阿米巴共培养时细菌包膜增大，这种影响取决于存在的寄生虫数量。即使不与棘阿米巴共培养，这种相互作用也会增强肺炎克雷伯菌后代的荚膜多糖产量。胶囊的高粘粘度使用沉淀测定法和串试验进行检测。我们还使用印度墨水染色筛选了其他肺炎克雷伯菌血清型，包括K1、K2、K5和K20，以与棘阿米巴相互作用，并发现了相同的相互作用效果。结论/意义：这些发现表明，棘阿米巴和肺炎克雷伯菌之间的相互作用可能会对公共卫生和医院疾病控制造成有害后果，尤其是高粘肺炎克雷伯菌感染。

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来源期刊

PLoS Neglected Tropical Diseases Medicine-Infectious Diseases

CiteScore

7.40

自引率

10.50%

发文量

723

审稿时长

2-3 weeks

期刊介绍： PLOS Neglected Tropical Diseases publishes research devoted to the pathology, epidemiology, prevention, treatment and control of the neglected tropical diseases (NTDs), as well as relevant public policy. The NTDs are defined as a group of poverty-promoting chronic infectious diseases, which primarily occur in rural areas and poor urban areas of low-income and middle-income countries. Their impact on child health and development, pregnancy, and worker productivity, as well as their stigmatizing features limit economic stability. All aspects of these diseases are considered, including: Pathogenesis Clinical features Pharmacology and treatment Diagnosis Epidemiology Vector biology Vaccinology and prevention Demographic, ecological and social determinants Public health and policy aspects (including cost-effectiveness analyses).