Method for Visualization of Emergency Granulopoiesis in the Zebrafish Embryo.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-08-01 Epub Date: 2023-06-12 DOI:10.1089/zeb.2023.0014

Ramizah Syahirah, Jennifer Beckman, Hanna Malik, Alan Y Hsu, Qing Deng

引用次数: 0

Abstract

Emergency granulopoiesis (EG) is a response to severe inflammation in which increased neutrophils are generated in the hematopoietic tissue. Photolabeling is utilized to distinguish newly developed neutrophils from existing neutrophils. However, this technique requires a strong laser line and labels subsets of the existing neutrophils. Here we create a transgenic zebrafish line that expresses a time-dependent switch from green fluorescent protein (GFP) to red fluorescent protein (RFP) in neutrophils, which allows quantification of EG using simple GFP/RFP ratiometric imaging.

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斑马鱼胚胎中紧急粒细胞生成的可视化方法。

急性粒细胞增多症（EG）是对严重炎症的反应，其中造血组织中产生的中性粒细胞增多。光标记用于区分新发展的中性粒细胞和现有的中性粒病毒。然而，这项技术需要一条强大的激光线，并标记现有中性粒细胞的子集。在这里，我们创建了一个转基因斑马鱼系，该系在中性粒细胞中表达从绿色荧光蛋白（GFP）到红色荧光蛋白（RFP）的时间依赖性转换，这允许使用简单的GFP/RFP比率成像来定量EG。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.