Method for Visualization of Emergency Granulopoiesis in the Zebrafish Embryo.

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2023-08-01 Epub Date: 2023-06-12 DOI:10.1089/zeb.2023.0014

Ramizah Syahirah, Jennifer Beckman, Hanna Malik, Alan Y Hsu, Qing Deng

引用次数: 0

Abstract

Emergency granulopoiesis (EG) is a response to severe inflammation in which increased neutrophils are generated in the hematopoietic tissue. Photolabeling is utilized to distinguish newly developed neutrophils from existing neutrophils. However, this technique requires a strong laser line and labels subsets of the existing neutrophils. Here we create a transgenic zebrafish line that expresses a time-dependent switch from green fluorescent protein (GFP) to red fluorescent protein (RFP) in neutrophils, which allows quantification of EG using simple GFP/RFP ratiometric imaging.

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斑马鱼胚胎中紧急粒细胞生成的可视化方法。

急性粒细胞增多症（EG）是对严重炎症的反应，其中造血组织中产生的中性粒细胞增多。光标记用于区分新发展的中性粒细胞和现有的中性粒病毒。然而，这项技术需要一条强大的激光线，并标记现有中性粒细胞的子集。在这里，我们创建了一个转基因斑马鱼系，该系在中性粒细胞中表达从绿色荧光蛋白（GFP）到红色荧光蛋白（RFP）的时间依赖性转换，这允许使用简单的GFP/RFP比率成像来定量EG。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.