Comparison of PD-L1 IHC 22C3 PharmDx Combined Positive Score (CPS) in Primary Versus Metastatic Nodal Squamous Cell Carcinomas of the Head and Neck: Is There a Significant Difference?

IF 1.3 4区 医学 Q3 ANATOMY & MORPHOLOGY
Ahmet Surucu, Tieying Hou, Matthew Kuhar, Greg Durm, Hector Mesa
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引用次数: 0

Abstract

PD-L1 IHC 22C3 pharmDx is an FDA-approved companion test to select patients for anti-PD-L1 immunotherapy. In head and neck squamous cell carcinoma PD-L1 expression is determined using a Combined Positive Score (CPS), which evaluates expression in tumor cells and tumor-associated leukocytes. We hypothesized that in nodal metastasis, the CPS should be higher given their inherent higher proportion of leukocytes. A significant difference in CPS between sites would mean that the tissue chosen for PD-L1 testing would impact patient eligibility for therapy. Currently, guidelines about which tissue should be tested do not exist. PD-L1 22C3 IHC was performed in the primary and nodal metastases of 35 head and neck squamous cell carcinoma, and a CPS was generated by 3 pathologists. Mean CPS was higher at the primary than the nodal metastasis: 47.2 versus 42.2; however, the difference was not statistically significant: P=0.259 . By therapeutic groups: negative (CPS <1), low (CPS 1-19) and high (CPS≥20), low-expression was more common in the primary: 40 vs. 26%, and high-expression in the nodal metastasis: 74 vs. 60% but this difference was not statistically significant: P=0.180. Stratified by positive versus negative (CPS <1 vs. ≥1), there were no differences between sites. Interobserver agreement for CPS among the 3 raters was slight for both sites: ƙ = 0.117 and 0.025, fair if stratified by therapeutic group: ƙ = 0.371 and 0.318, and near perfect if stratified as negative versus positive: ƙ = 0.652 and 1. There were no statistically significant differences in CPS between primary and nodal metastases independent of how the CPS was stratified.

PD-L1 IHC 22C3 PharmDx联合阳性评分(CPS)在头颈部原发性和转移性结节性鳞状细胞癌中的比较:有显著差异吗?
PD-L1 IHC 22C3 pharmDx是美国食品药品监督管理局批准的一项配套测试,用于选择患者进行抗PD-L1免疫治疗。在头颈部鳞状细胞癌中,PD-L1的表达是使用联合阳性评分(CPS)来确定的,该评分评估肿瘤细胞和肿瘤相关白细胞中的表达。我们假设,在淋巴结转移中,CPS应该更高,因为它们固有的白细胞比例更高。位点之间CPS的显著差异意味着选择用于PD-L1测试的组织将影响患者的治疗资格。目前,关于哪些组织应该接受检测的指导方针还不存在。PD-L1 22C3 IHC在35例头颈部鳞状细胞癌的原发性和淋巴结转移中进行,3名病理学家生成了CPS。原发性转移的平均CPS高于淋巴结转移:47.2比42.2;然而,差异无统计学意义:P=0.259。按治疗组分:阴性(CPS
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来源期刊
Applied Immunohistochemistry & Molecular Morphology
Applied Immunohistochemistry & Molecular Morphology ANATOMY & MORPHOLOGY-MEDICAL LABORATORY TECHNOLOGY
CiteScore
3.20
自引率
0.00%
发文量
153
期刊介绍: ​Applied Immunohistochemistry & Molecular Morphology covers newly developed identification and detection technologies, and their applications in research and diagnosis for the applied immunohistochemist & molecular Morphologist. Official Journal of the International Society for Immunohistochemisty and Molecular Morphology​.
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