Multilocus sequence analysis (MLSA) of a Nocardia cyriacigeorgica strain causing severe bovine mastitis in Bosnia and Herzegovina.

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Acta veterinaria Hungarica Pub Date : 2023-09-07 Print Date: 2023-10-17 DOI:10.1556/004.2023.00893

Oliver Stevanović, Dubravka Milanov, Isidora Prošić, Vladimir Gajdov, Drago Nedić, Željko Sladojević, Andrea Radalj

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引用次数: 0

Abstract

Nocardia cyriacigeorgica is a well-known agent of human nocardiosis and is considered an emerging pathogen, however, its identification to the species level is complex for many clinical laboratories. Available data on the clinical significance of N. cyriacigeorgica in veterinary medicine are sparse and mainly concern isolated reports of pyogranulomatous lesions in domestic animals. We report a case of severe bovine mastitis caused by N. cyriacigeorgica that did not respond to conventional antimicrobial therapy in a small holding in Bosnia and Herzegovina. After isolation of the pathogen, further identification by routine microbiological methods was not possible. Susceptibility to antimicrobials was tested using the disc diffusion method according to published recommendations. The sample was also tested by MALDI-ToF MS with inconclusive results. In addition, 16S rRNA sequence analysis, verified by multilocus sequence analysis (MLSA) using the gyrB, 16S rRNA, secA1, and hsp65 sequences, confirmed the species N. cyriacigeorgica. To our knowledge, this is the first report of isolation of N. cyriacigeorgica from a clinical case of bovine mastitis in a European dairy farm and the first MLSA method approach to distinguish a Nocardia spp. strain isolated from animals.

查看原文本刊更多论文

波斯尼亚和黑塞哥维那一株引起严重乳腺炎的cyriacigeorgica诺卡氏菌菌株的多基因座序列分析（MLSA）。

cyriacigeorgica诺卡氏菌是一种众所周知的人类诺卡氏病病原体，被认为是一种新出现的病原体，然而，对于许多临床实验室来说，其物种水平的鉴定是复杂的。关于N.cyriacigeorgica在兽医学中的临床意义的现有数据很少，主要涉及家畜化脓性肉芽肿性病变的孤立报道。我们报告了一例由N.cyriacigeorgica引起的严重乳腺炎，该病例在波斯尼亚和黑塞哥维那的一个小农场对常规抗菌治疗没有反应。在分离出病原体后，无法通过常规微生物学方法进行进一步鉴定。根据已发表的建议，使用椎间盘扩散法检测对抗菌药物的敏感性。样品还通过MALDI ToF MS进行了测试，结果不确定。此外，通过使用gyrB、16S rRNA、secA1和hsp65序列的多点序列分析（MLSA）验证的16S rNA序列分析证实了N.cyriacigeorgica物种。据我们所知，这是第一份从欧洲奶牛场的一例乳腺炎临床病例中分离出N.cyriacigeorgica的报告，也是第一份用MLSA方法区分诺卡氏菌的方法。从动物中分离出菌株。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta veterinaria Hungarica 农林科学-兽医学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： Acta Veterinaria Hungarica publishes original research papers presenting new scientific results of international interest, and to a limited extent also review articles and clinical case reports, on veterinary physiology (physiological chemistry and metabolism), veterinary microbiology (bacteriology, virology, immunology, molecular biology), on the infectious diseases of domestic animals, on veterinary parasitology, pathology, clinical veterinary science and reproduction.