Clinical and pathological correlation between urinary exosome miR-223 and IgAN patients.

IF 1.1 4区医学 Q3 UROLOGY & NEPHROLOGY

Clinical nephrology Pub Date : 2023-11-01 DOI:10.5414/CN110810

Jianling Song, Xinfang Qin, Hong Li, Guxiang Huang, Huixin Bi

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引用次数: 0

Abstract

Objective: To investigate the association between urine exosome miR-223 and clinical markers with pathological severity of IgA nephropathy (IgAN) in order to offer a new perspective for the evaluation of IgAN patients.

Materials and methods: Western blotting and transmission electron microscopy were used to identify the exosomes collected and isolated from subjects' urine. qRT-PCR was then performed to determine the expression level of miR-223. Following that, the relationship between miR-223 expression, clinical markers, and the severity of pathology in IgAN patients was examined.

Results: (1) Urine can be used to isolate exosomes since its marker protein was visible by Western blotting, and its size and structure were observable using transmission electron microscopy. (2) Expression levels of miR-223 in urinary exosomes were much higher in IgAN patients than in healthy subjects, and these were also positively correlated with creatinine (Cr) (rho = 0.396; p = 0.006), blood urea nitrogen (BUN) (rho = 0.371; p = 0.011), 24-hour urinary microalbumin (24hU-mALB) (rho = 0.341; p = 0.036), mesangial cell proliferation (rho = 0.359; p = 0.014), glomerular segmental sclerosis (rho = 0.417; p = 0.004), cell/fibroblast crescents (rho = 0.612; p = 0.000), glomerulosclerosis, and renal interstitial fibrosis (rho = 0.331; p = 0.025).

Conclusion: In urine exosomes, miR-223 might be considered a non-invasive biomarker for the assessment of IgAN disease progression.

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尿外泌体miR-223与IgAN患者的临床和病理相关性。

目的：探讨尿外泌体miR-223和临床标志物与IgA肾病（IgAN）病理严重程度的关系，为IgAN患者的评价提供新的视角。材料和方法：使用蛋白质印迹和透射电子显微镜鉴定从受试者尿液中收集和分离的外泌体。然后进行qRT-PCR以确定miR-223的表达水平。随后，研究了miR-223的表达、临床标志物和IgAN患者病理严重程度之间的关系。结果：（1）尿液可用于分离外泌体，因为其标记蛋白通过蛋白质印迹可见，并且其大小和结构通过透射电子显微镜可观察到。（2） IgAN患者尿外泌体中miR-223的表达水平远高于健康受试者，并且这些表达水平还与肌酐（Cr）（rho=0.396；p=0.006）、血尿素氮（BUN）（rho=0.371；p=0.011）、24小时尿微量白蛋白（24hU mALB）（rho=0.341；p=0.036）、系膜细胞增殖（rho0.359；p=0.014）呈正相关，肾小球节段性硬化（rho=0.417；p=0.004）、细胞/成纤维细胞新月体（rho0.612；p=0.000）、肾小球硬化和肾间质纤维化（rho0.331；p=0.025）。结论：在尿液外泌体中，miR-223可能被认为是评估IgAN疾病进展的非侵入性生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical nephrology 医学-泌尿学与肾脏学

CiteScore

2.10

自引率

9.10%

发文量

138

审稿时长

4-8 weeks

期刊介绍： Clinical Nephrology appears monthly and publishes manuscripts containing original material with emphasis on the following topics: prophylaxis, pathophysiology, immunology, diagnosis, therapy, experimental approaches and dialysis and transplantation.