Validation of an Innovative Chromatographic Method for Hypericin Quantification in Nanostructured Lipid Carriers.

IF 1.7 4区农林科学 Q3 CHEMISTRY, ANALYTICAL

Journal of AOAC International Pub Date : 2023-11-02 DOI:10.1093/jaoacint/qsad100

Patricia Rocha de Araújo, Mariana Rillo Sato, Marcela Tavares Luiz, Marlus Chorilli

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引用次数: 0

Abstract

Background: Hypericin (HYP) is a natural compound widely used as a food supplement. The encapsulation of HYP into nanosystems, such as nanostructured lipid carriers (NLC), is a promising strategy for delivering this lipophilic molecule and protecting it from degradation.

Objective: This study aims to develop and validate an analytical method to quantify the encapsulation efficiency of HYP in NLC.

Method: A reverse-phase high-performance liquid chromatography (HPLC) method was developed and validated according to the International Conference on Harmonization (ICH) guide Q2 (R1). NLC was prepared through the ultrasonication method, and HYP encapsulation efficiency was evaluated using the validated method.

Results: Separation was achieved using an isocratic mobile phase composed of acetonitrile, methanol, and ammonium acetate buffer (10 mM, pH 5.0) (54:36:10, v/v/v) and a reverse stationary phase. The specificity, linearity, precision, accuracy, and robustness of the method were assessed and confirmed during the validation. Furthermore, the validated method was able to determine the encapsulation efficiency of HYP in NLC.

Conclusions: The HPLC method was validated, and the results indicated the ability of NLC to deliver HYP compounds for further application as a food supplement.

Highlights: HYP is used as a food supplement and for photodynamic therapy (PDT). The developed method was specific, linear, precise, accurate, and robust. NLCs showed a high ability to encapsulate HYP.

查看原文本刊更多论文

纳米结构脂质载体中金丝桃素定量的创新色谱方法的验证。

背景：金丝桃素是一种广泛用作食品补充剂的天然化合物。将HYP封装到纳米系统中，如纳米结构脂质载体（NLC），是递送这种亲脂性分子并保护其免受降解的一种很有前途的策略。目的：本研究旨在开发和验证一种定量HYP在NLC中包封效率的分析方法。方法：根据国际协调会议（ICH）指南Q2（R1），开发并验证了反相高效液相色谱（HPLC）方法。通过超声法制备了NLC，并使用验证的方法评估了HYP的包封效率。结果：使用由乙腈、甲醇和乙酸铵缓冲液（10 mM，pH 5.0）（54:36:10，v/v/v）和反向固定相。在验证过程中对该方法的特异性、线性、精密度、准确性和稳健性进行了评估和确认。此外，经验证的方法能够测定HYP在NLC中的包封效率。结论：高效液相色谱法得到了验证，结果表明NLC具有递送HYP化合物的能力，可作为食品补充剂进一步应用。亮点：HYP被用作食品补充剂和光动力疗法（PDT）。所开发的方法具有特异性、线性、精确性、准确性和稳健性。NLCs表现出很高的封装HYP的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of AOAC International 医学-分析化学

CiteScore

3.10

自引率

12.50%

发文量

144

审稿时长

2.7 months

期刊介绍： The Journal of AOAC INTERNATIONAL publishes the latest in basic and applied research in analytical sciences related to foods, drugs, agriculture, the environment, and more. The Journal is the method researchers'' forum for exchanging information and keeping informed of new technology and techniques pertinent to regulatory agencies and regulated industries.