Urinary MicroRNAs, Possible Biomarkers for Early Detection of Patients with Autosomal Dominant Polycystic Kidney Disease (ADPKD).

IF 0.8 4区医学 Q4 UROLOGY & NEPHROLOGY

Iranian journal of kidney diseases Pub Date : 2023-03-01

Yaghoob Ghasemi, Alireza Mardomi, Effat Alizadeh, Hamid Tayebi Khosroshahi

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引用次数: 0

Abstract

Introduction: Autosomal dominant polycystic kidney disease (ADPKD) is a prevalent renal disorder that causes abnormal growth of renal epithelial cells. The excessive expansion of renal epithelial cells can lead to cyst formation that is associated with serious renal complications. The early diagnosis of ADPKD makes the control of the disease somehow attainable. Regarding the diagnostic potential of microRNAs (miRs) as robust clinical biomarkers, the present study aimed to examine the potential of urinary miRs in early diagnosis of ADPKD in asymptomatic patients.

Methods: Urine samples were obtained from 20 asymptomatic ADPKD patients and 20 healthy control individuals and the miR content of the samples was extracted and converted to cDNA for the qRT-PCR experiment. The relative expressions of miR-17, miR-21, miR-143, and miR-223 were evaluated in ADPKD cases and healthy individuals. Serum levels of kidney function markers were also evaluated in the study participants.

Results: The urine samples of patients with ADPKD demonstrated higher levels of miR-17, miR-21, and miR-143 along with a lower miR-223 level compared to the healthy control group.

Conclusion: This study revealed the differential expression of the studied miRs in ADPKD patients. Detection of miRs in urinary samples might provide a useful platform for early diagnosis of ADPKD in asymptomatic patients. DOI: 10.52547/ijkd.7281.

本刊更多论文

尿微rna，常染色体显性多囊肾病(ADPKD)患者早期检测的可能生物标志物。

常染色体显性多囊肾病(ADPKD)是一种常见的肾脏疾病，引起肾上皮细胞异常生长。肾上皮细胞的过度扩张可导致囊肿形成，并伴有严重的肾脏并发症。ADPKD的早期诊断使得控制这种疾病在某种程度上是可能的。鉴于microRNAs (miRs)作为强大的临床生物标志物的诊断潜力，本研究旨在研究尿液miRs在无症状患者ADPKD早期诊断中的潜力。方法:取20例无症状ADPKD患者和20例健康对照者尿液标本，提取miR含量转化为cDNA，进行qRT-PCR实验。在ADPKD病例和健康个体中评估miR-17、miR-21、miR-143和miR-223的相对表达。同时还对研究参与者的血清肾功能指标进行了评估。结果:与健康对照组相比，ADPKD患者的尿液样本显示miR-17、miR-21和miR-143水平较高，miR-223水平较低。结论:本研究揭示了所研究的miRs在ADPKD患者中的差异表达。尿液样本中mir的检测可能为无症状患者ADPKD的早期诊断提供一个有用的平台。DOI: 10.52547 / ijkd.7281。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian journal of kidney diseases UROLOGY & NEPHROLOGY-

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Iranian Journal of Kidney Diseases (IJKD), a peer-reviewed journal in English, is the official publication of the Iranian Society of Nephrology. The aim of the IJKD is the worldwide reflection of the knowledge produced by the scientists and clinicians in nephrology. Published quarterly, the IJKD provides a new platform for advancement of the field. The journal’s objective is to serve as a focal point for debates and exchange of knowledge and experience among researchers in a global context. Original papers, case reports, and invited reviews on all aspects of the kidney diseases, hypertension, dialysis, and transplantation will be covered by the IJKD. Research on the basic science, clinical practice, and socio-economics of renal health are all welcomed by the editors of the journal.