Comprehensive Characterization and Identification of Chemical Constituents of Xiangsha Pingwei Pills by UPLC-Q-TOF-MS.

IF 1.7 4区 农林科学 Q3 CHEMISTRY, ANALYTICAL
Hui Xiong, Na Li, Yongzhou Yu, Zi Liu, Zhe Li, Lanqingqing Zhao
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引用次数: 0

Abstract

Background: Xiangsha Pingwei Pills (XPP) is a traditional Chinese medicine (TCM) prescription, which is widely used to treat epigastric pain in China. Its systematic chemical characteristics have rarely been reported, which hinders the interpretation of the material basis of its prescription.

Objective: To establish a rapid and effective component characterization method for XPP using ultra-HPLC-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) and the data post-processing program, Peakview 1.2 software.

Methods: A UPLC-Q-TOF-MS method coupled with Peakview 1.2 software was successfully established for the first time to investigate the complex constituents of XPP. Accurate MS and MS/MS data were detected in positive and negative ion mode. The compounds were tentatively identified based on their retention times, MS, and MS/MS data, as well as reference standards and from the literature.

Results: The chemical profile of XPP was acquired, and a total of 130 compounds in XPP were preliminarily identified for the first time, including 6 organic acids, 59 flavonoids, 13 lignans, 20 terpenoids, 9 phenylpropanoids, 6 alkaloids, 5 amino acids, and 12 other compounds.

Conclusion: A rapid and effective UPLC-Q-TOF-MS method for the main chemical components of XPP has been established for further characterizing constituents and the overall quality control of XPP.

Highlights: This is the first report of a comprehensive analysis method for the main chemical components of XPP, which aims to lay a solid foundation for the chemical basis and overall quality control of XPP.

用UPLC-Q-TOF-MS对香沙平胃丸的化学成分进行了综合表征和鉴定。
背景:香沙平胃丸是一种中药方剂,在中国被广泛用于治疗胃脘痛。其系统的化学特性很少被报道,这阻碍了对其处方的物质基础的解释。目的:利用超高效液相色谱-四极杆飞行时间串联质谱(UPLC-Q-TOF-MS)和数据后处理程序Peakview 1.2软件建立快速有效的XPP成分表征方法。方法:首次建立了UPLC-Q-TOF-MS联用Peakview 1.2软件对XPP的复杂成分进行分析。在正离子和负离子模式下检测准确的MS和MS/MS数据。根据化合物的保留时间、质谱、质谱/质谱数据、参考标准品和文献初步鉴定化合物。结果:获得了XPP的化学图谱,首次初步鉴定出XPP中共130个化合物,包括6种有机酸、59种黄酮类化合物、13种木脂素、20种萜类化合物、9种苯丙素、6种生物碱、5种氨基酸和12种其他化合物。结论:建立了一种快速有效的XPP主要化学成分的UPLC-Q-TOF-MS分析方法,可进一步对XPP进行成分表征和整体质量控制。重点:本报告首次对XPP主要化学成分进行综合分析,为XPP的化学基础和整体质量控制打下坚实的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of AOAC International
Journal of AOAC International 医学-分析化学
CiteScore
3.10
自引率
12.50%
发文量
144
审稿时长
2.7 months
期刊介绍: The Journal of AOAC INTERNATIONAL publishes the latest in basic and applied research in analytical sciences related to foods, drugs, agriculture, the environment, and more. The Journal is the method researchers'' forum for exchanging information and keeping informed of new technology and techniques pertinent to regulatory agencies and regulated industries.
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