Serum antibodies to selected Helicobacter pylori antigens are associated with active gastritis in patients seen at the University Teaching Hospital in Lusaka, Zambia.
IF 1.2 4区 医学Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Violet Kayamba, Julia Butt, Matthew Gordon Varga, Aaron Shibemba, Maria Blanca Piazuelo, Keith Tucker Wilson, Kanekwa Zyambo, Simutanyi Mwakamui, Chola Mulenga, Tim Waterboer, Meira Epplein, Douglas Corbett Heimburger, Masharip Atadzhanov, Paul Kelly
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引用次数: 1
Abstract
Introduction: Little is known about specific bacterial characteristics of Helicobacter pylori (H. pylori) infection influencing gastric carcinogenesis in Zambia. The aim of this study was to evaluate the associations between pre-selected H. pylori antibodies with gastric cancer, premalignant lesions and active gastritis.
Methods: This was cross-sectional study with multiple comparisons of patients with gastric cancer (GC), gastric premalignant (GP) lesions and active or chronic gastritis. A fluorescent bead-based antibody multiplex serology assay was used to quantify antibodies to thirteen immunogenic H. pylori antigens. Logistic regression models were used to examine the associations.
Results: Included were 295 patients with: 59 GC, 27 GP lesions, 48 active and 161 chronic gastritis. Overall, 257/295 (87%) were H. pylori positive. H. pylori seropositivity was not associated with sex, age, body mass index, socio-economic status, HIV infection, alcohol consumption or cigarette smoking (p-values all above 0.05). When compared to the patients with chronic gastritis, the presence of catalase and cinnamyl alcohol dehydrogenase (Cad) antibodies was positively associated with GP lesions (OR 3.53; 95% CI 1.52-8.17 and OR 2.47; 95% CI 1.08-5.67 respectively). However, seropositivity to Cad antibodies was significantly lower in GC patients (OR 0.28; 95% CI 0.09-0.83). Compared to chronic, active gastritis was significantly associated with (p<0.05) H. pylori sero-positivity (OR 9.46; 95% CI 1.25-71.52) and specific antibodies including cytotoxin-associated gene A, vacuolating cytotoxin A, Helicobacter cysteine-rich protein C, hypothetical protein HP0305 and outer membrane protein HP1564.
Conclusions: Among Zambian patients seen at a single center, antibodies to H. pylori (CagA, VacA, Omp, HcpC, HP0305 and HpaA) were associated with active gastritis.
导读:目前对赞比亚幽门螺杆菌感染影响胃癌发生的具体细菌特征知之甚少。本研究的目的是评估预先选择的幽门螺杆菌抗体与胃癌、癌前病变和活动性胃炎之间的关系。方法:这是一项横断面研究，对胃癌(GC)、胃癌前病变(GP)和活动性或慢性胃炎患者进行多重比较。采用基于荧光珠的抗体多重血清学方法定量测定13种免疫原性幽门螺杆菌抗原的抗体。使用逻辑回归模型来检验相关性。结果:纳入295例患者，其中GC 59例，GP 27例，活动性48例，慢性胃炎161例。总体而言，257/295例(87%)幽门螺杆菌阳性。幽门螺杆菌血清阳性与性别、年龄、体重指数、社会经济地位、HIV感染、饮酒或吸烟无关(p值均大于0.05)。与慢性胃炎患者相比，过氧化氢酶和肉桂醇脱氢酶(Cad)抗体的存在与GP病变呈正相关(OR 3.53;95% CI 1.52-8.17, OR 2.47;95% CI分别为1.08-5.67)。然而，GC患者血清Cad抗体阳性显著降低(OR 0.28;95% ci 0.09-0.83)。与慢性胃炎相比，活动性胃炎与幽门螺杆菌血清阳性显著相关(OR 9.46;95% CI 1.25-71.52)和特异性抗体，包括细胞毒素相关基因A、液泡细胞毒素A、富含半胱氨酸的幽门螺杆菌蛋白C、假设蛋白HP0305和外膜蛋白HP1564。结论:在同一中心就诊的赞比亚患者中，幽门螺杆菌抗体(CagA、VacA、Omp、HcpC、HP0305和HpaA)与活动性胃炎相关。
期刊介绍：
Driven and guided by the priorities articulated in the Malawi National Health Research Agenda, the Malawi Medical Journal publishes original research, short reports, case reports, viewpoints, insightful editorials and commentaries that are of high quality, informative and applicable to the Malawian and sub-Saharan Africa regions. Our particular interest is to publish evidence-based research that impacts and informs national health policies and medical practice in Malawi and the broader region.
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