mRNA expression of immune factors by milk somatic cells from healthy Holstein lactating cows.

IF 1.1 4区 农林科学 Q2 Veterinary
Kenji Murakami, Tomochika Fukuhara, Shunsuke Kure, Takaaki Shimosakai, Ayano Sato, Ryo Murata, Keigo Kosenda, Hiromichi Ohtsuka
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引用次数: 0

Abstract

This study investigated the mRNA of immune factors expressed by milk somatic cells from 72 healthy lactating Holstein cows on 1 farm. Milk samples were collected aseptically from the right front mammary gland before milking. The milk samples that had a negative reaction to the California mastitis test were used to analyze the mRNA of immune factors. Cows were divided into 2 groups based on the detection of bacteria in milk samples: positive group (n = 22 cows), which showed bacteria in cultures, and negative group (n = 50 cows), which did not show bacteria in cultures. There were significant positive correlations among the relative mRNA levels of interleukin (IL)-6, IL-8, arginase 1, chemokine (C-C motif) ligand (CCL) 1, and chemokine (C-X-C motif) ligand (CXCL) 13, as well as among the relative mRNA levels of IL-10, pentraxin 3, CCL5, and CCL14. Significantly high levels of IL-1β, IL-6, IL-8, arginase 1, Batf, CCL1, CXCL14, and toll-like receptor 4 in the positive group were discovered compared to the negative group. These results suggest that the presence of bacteria in lactating healthy dairy cows may affect mRNA levels of inflammatory mediators expressed by somatic cells.

健康荷斯坦泌乳奶牛乳体细胞免疫因子mRNA的表达。
本研究对1个农场72头健康泌乳荷斯坦奶牛乳体细胞表达的免疫因子mRNA进行了研究。挤奶前从右前乳腺无菌采集乳样。对加州乳腺炎试验阴性的牛奶样品进行免疫因子mRNA分析。根据牛奶样品中细菌的检测情况将奶牛分为培养物中有细菌的阳性组(n = 22头)和培养物中没有细菌的阴性组(n = 50头)。白细胞介素(IL)-6、IL-8、精氨酸酶1、趋化因子(C-X-C motif)配体(CCL) 1、趋化因子(C-X-C motif)配体(CXCL) 13的相对mRNA水平与IL-10、戊烷素3、CCL5、CCL14的相对mRNA水平呈显著正相关。与阴性组相比,阳性组IL-1β、IL-6、IL-8、精氨酸酶1、Batf、CCL1、CXCL14和toll样受体4水平显著升高。上述结果提示,健康泌乳奶牛体内细菌的存在可能影响体细胞表达的炎症介质mRNA水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
58
审稿时长
>24 weeks
期刊介绍: The Canadian Journal of Veterinary Research, published by the Canadian Veterinary Medical Association, is Canada''s only veterinary research publication. This quarterly peer-reviewed online-only journal has earned a wide international readership through the publishing of high quality scientific papers in the field of veterinary medicine. The Journal publishes the results of original research in veterinary and comparative medicine.
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