Acarbose Mitigates Age-Dependent Alterations in Erythrocyte Membrane Transporters During Aging in Rats.

IF 2.2 4区医学 Q3 GERIATRICS & GERONTOLOGY

Rejuvenation research Pub Date : 2023-08-01 Epub Date: 2023-06-12 DOI:10.1089/rej.2023.0010

Jitendra Kumar Arya, Raushan Kumar, Akanksha Singh, Parisha Srivastava, Arun Kumar Yadawa, Syed Ibrahim Rizvi

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引用次数: 0

Abstract

Acarbose (ACA), a well-studied and effective inhibitor of α-amylase and α-glucosidase, is a postprandial-acting antidiabetic medicine. The membrane of the erythrocyte is an excellent tool for analyzing different physiological and biochemical activities since it experiences a range of metabolic alterations throughout aging. It is uncertain if ACA modulates erythrocyte membrane activities in an age-dependent manner. As a result, the current study was conducted to explore the influence of ACA on age-dependent deteriorated functions of transporters/exchangers, disrupted levels of various biomarkers such as lipid hydroperoxides (LHs), protein carbonyl (PCO), sialic acid (SA), total thiol (-SH), and erythrocyte membrane osmotic fragility. In addition to a concurrent increase in Na⁺/H⁺ exchanger activity and concentration of LH, PCO, and osmotic fragility, we also detected a considerable decrease in membrane-linked activities of Ca²⁺-ATPase (PMCA) and Na⁺/K⁺-ATPase (NKA), as well as concentrations of SA and -SH in old-aged rats. The aging-induced impairment of the activities of membrane-bound ATPases and the changed levels of redox biomarkers were shown to be effectively restored by ACA treatment.

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阿卡波糖缓解大鼠衰老过程中红细胞膜转运体随年龄发生的变化

阿卡波糖（ACA）是一种经过充分研究的有效的α-淀粉酶和α-葡萄糖苷酶抑制剂，是一种餐后作用的抗糖尿病药物。红细胞膜是分析不同生理和生化活动的绝佳工具，因为它在整个衰老过程中会经历一系列新陈代谢变化。目前还不确定 ACA 是否以年龄依赖的方式调节红细胞膜的活动。因此，本研究旨在探讨 ACA 对随年龄增长而退化的转运体/交换体功能、各种生物标志物（如脂质氢过氧化物（LHs）、蛋白质羰基（PCO）、硅铝酸（SA）、总硫醇（-SH））和红细胞膜渗透脆性的影响。除了 Na+/H+ 交换器活性以及 LH、PCO 和渗透脆性的浓度同时升高外，我们还检测到老年大鼠与膜相连的 Ca2+-ATP 酶（PMCA）和 Na+/K+-ATP 酶（NKA）的活性以及 SA 和 -SH 的浓度显著降低。经 ACA 治疗后，老化引起的膜结合 ATP 酶活性减弱和氧化还原生物标志物水平的变化得到了有效恢复。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rejuvenation research 医学-老年医学

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Rejuvenation Research publishes cutting-edge, peer-reviewed research on rejuvenation therapies in the laboratory and the clinic. The Journal focuses on key explorations and advances that may ultimately contribute to slowing or reversing the aging process, and covers topics such as cardiovascular aging, DNA damage and repair, cloning, and cell immortalization and senescence. Rejuvenation Research coverage includes: Cell immortalization and senescence Pluripotent stem cells DNA damage/repair Gene targeting, gene therapy, and genomics Growth factors and nutrient supply/sensing Immunosenescence Comparative biology of aging Tissue engineering Late-life pathologies (cardiovascular, neurodegenerative and others) Public policy and social context.