Claudin expression in pulmonary adenoid cystic carcinoma and mucoepidermoid carcinoma.

IF 2.3 4区 医学 Q3 ONCOLOGY
Pathology & Oncology Research Pub Date : 2023-08-09 eCollection Date: 2023-01-01 DOI:10.3389/pore.2023.1611328
Marton Gyulai, Tunde Harko, Katalin Fabian, Luca Karsko, Laszlo Agocs, Balazs Szigeti, Janos Fillinger, Zoltan Szallasi, Orsolya Pipek, Judit Moldvay
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引用次数: 0

Abstract

Background: Although the expression of tight junction protein claudins (CLDNs) is well known in common histological subtypes of lung cancer, it has not been investigated in rare lung cancers. The aim of our study was to examine the expression of different CLDNs in pulmonary salivary gland tumors. Methods: 35 rare lung cancers including pathologically confirmed 12 adenoid cystic carcinomas (ACCs) and 23 mucoepidermoid carcinomas (MECs) were collected retrospectively. Immunohistochemical (IHC) staining was performed on formalin fixed paraffin embedded (FFPE) tumor tissues, and CLDN1, -2, -3, -4, -5, -7, and -18 protein expressions were analyzed. The levels of immunopositivity were determined with H-score. Certain pathological characteristics of ACC and MEC samples (tumor grade, presence of necrosis, presence of blood vessel infiltration, and degree of lymphoid infiltration) were also analyzed. Results: CLDN overexpression was observed in both tumor types, especially in CLDN2, -7, and -18 IHC. Markedly different patterns of CLDN expression were found for ACC and MEC tumors, especially for CLDN1, -2, -4, and -7, although none of these trends remained significant after correction for multiple testing. Positive correlations between expressions of CLDN2 and -5, CLDN3 and -4, and CLDN5 and -18 were also demonstrated. Tumors of never-smokers presented lower levels of CLDN18 than tumors of current smokers (p-value: 0.003). Conclusion: This is the first study to comprehensively describe the expression of different CLDNs in lung ACC and MEC. Overexpression of certain CLDNs may pave the way for targeted anti-claudin therapy in these rare histological subtypes of lung cancer.

Abstract Image

Abstract Image

Abstract Image

Claudin在肺腺样囊性癌和粘液表皮样癌中的表达。
背景:尽管紧密连接蛋白claudins(CLDNs)在常见的癌症组织学亚型中的表达是众所周知的,但在罕见的肺癌中尚未进行研究。我们研究的目的是检测不同CLDN在肺唾液腺肿瘤中的表达。方法:对35例罕见肺癌进行回顾性分析,其中经病理证实的腺样囊性癌12例,黏液表皮样癌23例。对福尔马林固定石蜡包埋(FFPE)肿瘤组织进行免疫组织化学(IHC)染色,分析CLDN1、-2、-3、-4、-5、-7和-18蛋白的表达。免疫阳性水平用H-core测定。还分析了ACC和MEC样本的某些病理特征(肿瘤分级、坏死、血管浸润和淋巴浸润程度)。结果:在两种肿瘤类型中都观察到CLDN过表达,尤其是在CLDN2、-7和-18 IHC中。ACC和MEC肿瘤的CLDN表达模式明显不同,尤其是CLDN1、-2、-4和-7,尽管经过多次检测校正后,这些趋势都不显著。CLDN2和-5、CLDN3和-4以及CLDN5和-18的表达也呈正相关。从不吸烟的肿瘤的CLDN18水平低于现在吸烟的肿瘤(p值:0.003)。结论:这是第一项全面描述不同CLDN在肺ACC和MEC中表达的研究。某些CLDN的过度表达可能为这些罕见的癌症组织学亚型的靶向抗凝血素治疗铺平道路。
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来源期刊
CiteScore
6.30
自引率
0.00%
发文量
134
审稿时长
4-8 weeks
期刊介绍: Pathology & Oncology Research (POR) is an interdisciplinary Journal at the interface of pathology and oncology including the preclinical and translational research, diagnostics and therapy. Furthermore, POR is an international forum for the rapid communication of reviews, original research, critical and topical reports with excellence and novelty. Published quarterly, POR is dedicated to keeping scientists informed of developments on the selected biomedical fields bridging the gap between basic research and clinical medicine. It is a special aim for POR to promote pathological and oncological publishing activity of colleagues in the Central and East European region. The journal will be of interest to pathologists, and a broad range of experimental and clinical oncologists, and related experts. POR is supported by an acknowledged international advisory board and the Arányi Fundation for modern pathology.
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