COMPARISON OF 2D AND 3D PRIMARY CELL CULTURES OBTAINED FROM EXPLANT OF HIGH-GRADE UROTHELIAL BLADDER CANCER.

Q3 Medicine

Experimental oncology Pub Date : 2023-06-26 DOI:10.15407/exp-oncology.2023.01.130

L V Garmanchuk, P G Yakovlev, G V Ostrovska, Yu A Stupak, O V Skachkova, O I Gorbach, Yu V Stepanov

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引用次数: 0

Abstract

Studying the biological characteristics of bladder cancer in primary culture can be an effective way for diagnostic and prognostic purposes, as well as choosing a scheme for personalized therapy.

Aim: To characterize and compare 2D and 3D primary cell cultures obtained from the same tumor sample resected from a patient with high-grade bladder cancer.

Materials and methods: 2D and 3D primary cell cultures were obtained from explants of resected bladder cancer. Glucose metabolism, lactate dehydrogenase (LDH) activity, and level of apoptosis were studied.

Results: Multicellular tumor spheroids (3D) differ from planar culture (2D) by more pronounced consumption of glucose from the culture medium (1.7 times higher than 2D on Day 3 of culture), increased lactate dehydrogenase activity (2.5 times higher on Day 3 vs. Day 1 of cultivation, while in 2D culture LDH activity is constant), stronger acidification of the extracellular environment (pH dropped by 1 in 3D and by 0.5 in 2D). Spheroids demonstrate enhanced resistance to apoptosis (1.4 times higher).

Conclusion: This methodological technique can be used both for tumor characterization and for selection of optimal postoperative chemotherapeutic schemes.

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高级别尿路上皮性膀胱癌外植体二维与三维原代细胞培养的比较。

在原代培养中研究膀胱癌的生物学特性，可为膀胱癌的诊断和预后提供有效的方法，并可为患者选择个性化的治疗方案。目的:描述和比较从高级别膀胱癌患者切除的同一肿瘤样本中获得的2D和3D原代细胞培养物。材料和方法:对膀胱癌切除后的外植体进行二维和三维原代细胞培养。葡萄糖代谢、乳酸脱氢酶(LDH)活性及细胞凋亡水平进行研究。结果:多细胞肿瘤球体(3D)与平面培养(2D)的不同之处是，更明显地消耗了培养基中的葡萄糖(培养第3天比2D高1.7倍)，乳酸脱氢酶活性增加(第3天比培养第1天高2.5倍，而在2D培养中LDH活性不变)，细胞外环境酸化更强(pH在3D中下降1，在2D中下降0.5)。球状体对细胞凋亡的抗性增强(高1.4倍)。结论:该方法既可用于肿瘤的特征分析，也可用于选择最佳的术后化疗方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental oncology Medicine-Oncology

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The Experimental Oncology is an English-language journal that publishes review articles, original contributions, short communications, case reports and technical advances presenting new data in the field of experimental and fundamental oncology. Manuscripts should be written in English, contain original work, which has not been published or submitted for publication elsewhere. It also implies the transfer of the Copyright from the author to “Experimental Oncology”. No part of journal publications may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the prior permission of the publisher.