New progresses in the isolation of primary hepatic stellate cells

Yangyang Kong, Li-xia Liu
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Abstract

Hepatic stellate cells(HSCs), also called fat-storing cells or Ito cells, are one of inherent liver non-parenchymal cell types.They constitute<8% of the total liver cell population.They locate in the Disse space between liver sinusoidal endothelial cells and hepatocytes, and store Vitamin A in the form of lipid drops.HSCs are the main source of extracellular matrix.Their activation is the key process in the development of liver fibrosis.The isolation of primary HSCs is the basis of studying the mechanism of liver fibrosis.It is one of the research focus on the area of liver fibrosis.The methods of primary HSCs isolation include the classic two-step enzyme perfusion, fluorescence activated cell sorting and magnetic-activated cell-sorting.This review will summarize the new progresses of primary HSCs isolation in recent years. Key words: Hepatic stellate cells; Cell separation; Two-step enzyme perfusion; Fluorescence activated cell sorting; Magnetic-activated cell-sorting
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原代肝星状细胞分离的新进展
肝星状细胞(Hepatic stellate cells, hsc),又称脂肪储存细胞或Ito细胞,是肝脏固有的非实质细胞类型之一。它们占肝细胞总数的8%以下。它们位于肝窦内皮细胞和肝细胞之间的椎间盘间隙,以脂滴的形式储存维生素A。造血干细胞是细胞外基质的主要来源。它们的激活是肝纤维化发展的关键过程。原代造血干细胞的分离是研究肝纤维化机制的基础。它是肝纤维化领域的研究热点之一。原代造血干细胞的分离方法包括经典的两步酶灌注法、荧光活化细胞分选法和磁活化细胞分选法。本文就近年来分离原代造血干细胞的新进展作一综述。关键词:肝星状细胞;细胞分离;两步酶灌注;荧光活化细胞分选;Magnetic-activated cell-sorting
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