The Effect of Fingolimod on Renal Ischemia/Reperfusion Injury in a Rat Model.

IF 2.9 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current molecular pharmacology Pub Date : 2024-01-01 DOI:10.2174/1874467217666230825113406

Yasin Bagheri, Elham Ahmadian, Seyyedeh Mina Hejazian, Maryam Raeesi, Sepideh Zununi Vahed, Mohammadreza Ardalan

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引用次数: 0

Abstract

Background: Ischemia/reperfusion injury (IRI) is a leading cause of acute kidney injury (AKI) that induces inflammation and oxidative stress. The main goal of the current study was to assess the impact of fingolimod on kidney IRI in rats.

Methods: For this purpose, 18 male Wistar rats (220-250g) were divided into three groups including (i) Sham, (ii) I/R, and (iii) fingolimod+I/R. The last group was pretreated with a single dose of fingolimod (1mg/kg) (intraperitoneal injection) before induction of the I/R injury. Kidney function, oxidative stress marker (malondialdehyde), and antioxidant markers (catalase, superoxide dismutase, glutathione, glutathione peroxidase, and total antioxidant capacity) were determined in the kidney tissue of the rats. Moreover, kidney samples were taken for histological analysis.

Results: Fingolimod pre-treatment could significantly improve the glutathione peroxidase (p<0.01) and glutathione (p<0.001) activities along with the total antioxidant capacity levels (p<0.001) when compared to the I/R group. Moreover, significant recovery of kidney function and histology was seen in the fingolimod+ I/R group compared to the I/R group (p<0.01).

Conclusion: Fingolimod pretreatment could improve renal function, antioxidant capacity, and histological alterations after I/R injury. Hence, it might protect the kidney against IRI-related kidney damage including AKI and transplantation.

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芬戈莫德对大鼠肾缺血再灌注损伤的影响。

背景:缺血/再灌注损伤(IRI)是急性肾损伤(AKI)的主要原因，可诱导炎症和氧化应激。本研究的主要目的是评估芬戈莫德对大鼠肾IRI的影响。方法:雄性Wistar大鼠18只(220 ~ 250g)，分为Sham组、i /R组、fingolimod+ i /R组。最后一组大鼠在诱导I/R损伤前腹腔注射单剂量fingolimod (1mg/kg)。测定大鼠肾脏组织的肾功能、氧化应激标志物(丙二醛)和抗氧化标志物(过氧化氢酶、超氧化物歧化酶、谷胱甘肽、谷胱甘肽过氧化物酶和总抗氧化能力)。并取肾脏标本进行组织学分析。结果:芬戈莫德预处理可显著改善大鼠谷胱甘肽过氧化物酶活性(p)。结论:芬戈莫德预处理可改善I/R损伤后的肾功能、抗氧化能力及组织学改变。因此，它可能保护肾脏免受iri相关肾脏损害，包括AKI和移植。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current molecular pharmacology Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

4.90

自引率

3.70%

发文量

112

期刊介绍： Current Molecular Pharmacology aims to publish the latest developments in cellular and molecular pharmacology with a major emphasis on the mechanism of action of novel drugs under development, innovative pharmacological technologies, cell signaling, transduction pathway analysis, genomics, proteomics, and metabonomics applications to drug action. An additional focus will be the way in which normal biological function is illuminated by knowledge of the action of drugs at the cellular and molecular level. The journal publishes full-length/mini reviews, original research articles and thematic issues on molecular pharmacology. Current Molecular Pharmacology is an essential journal for every scientist who is involved in drug design and discovery, target identification, target validation, preclinical and clinical development of drugs therapeutically useful in human disease.