Hyperbaric oxygen and treadmill exercise partially prevented bone loss and bone microarchitecture deterioration in ovariectomized rats.

IF 0.8 4区医学 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Diving and hyperbaric medicine Pub Date : 2023-06-30 DOI:10.28920/dhm53.2.111-119

Xiaoling Peng, Binli Gao, Xiangxiu Wang, Xiaohong Qin, Mei Peng, Xianrong Zeng

{"title":"Hyperbaric oxygen and treadmill exercise partially prevented bone loss and bone microarchitecture deterioration in ovariectomized rats.","authors":"Xiaoling Peng, Binli Gao, Xiangxiu Wang, Xiaohong Qin, Mei Peng, Xianrong Zeng","doi":"10.28920/dhm53.2.111-119","DOIUrl":null,"url":null,"abstract":"Introduction: Previous studies have demonstrated the beneficial effects of treadmill exercise (EX) on osteoporosis, and of hyperbaric oxygen (HBO) on osteoblast and osteoclast formation in vitro. We investigated the effects of HBO and the combination of HBO and EX on osteoporosis in ovariectomized rats.Methods: Forty 3-month-old female Sprague-Dawley rats were randomly divided into 5 groups (n = 8): a sham control group (Control); an ovariectomy group; an ovariectomy with treadmill exercise treatment group; an ovariectomy with HBO treatment group; and an ovariectomy with HBO treatment combined with treadmill exercise group. The HBO exposures were 203 kPa, 85-90% O₂, 90 min and the exercise regimen was 20 m·min⁻¹, 40 min·day¹, 5° slope. Both treatments were administered once daily, five days a week for 12 weeks until the rats were sacrificed.Results: All three treatments (HBO, exercise, and both combined) significantly promoted the expression of the osteoblast-related gene and oxidative metabolism-related gene (PGC-1α). They also exerted significant inhibitory effects on the osteoclast-related mRNA expression (RANKL) and bone resorption marker CTX-I. Additionally, exercise and the combination exercise-HBO treatment increased serum superoxide dysmutase (SOD) and sclerostin expression. No significant between-group difference was observed.Conclusions: Hyperbaric oxygen, exercise, and the combination ameliorated bone microarchitecture deterioration and ovariectomy-induced bone loss in rats, and these inhibitory effects may be associated with the increased SOD and up-regulated PGC-1α.","PeriodicalId":11296,"journal":{"name":"Diving and hyperbaric medicine","volume":"53 2","pages":"111-119"},"PeriodicalIF":0.8000,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584393/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diving and hyperbaric medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.28920/dhm53.2.111-119","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Previous studies have demonstrated the beneficial effects of treadmill exercise (EX) on osteoporosis, and of hyperbaric oxygen (HBO) on osteoblast and osteoclast formation in vitro. We investigated the effects of HBO and the combination of HBO and EX on osteoporosis in ovariectomized rats.

Methods: Forty 3-month-old female Sprague-Dawley rats were randomly divided into 5 groups (n = 8): a sham control group (Control); an ovariectomy group; an ovariectomy with treadmill exercise treatment group; an ovariectomy with HBO treatment group; and an ovariectomy with HBO treatment combined with treadmill exercise group. The HBO exposures were 203 kPa, 85-90% O₂, 90 min and the exercise regimen was 20 m·min⁻¹, 40 min·day¹, 5° slope. Both treatments were administered once daily, five days a week for 12 weeks until the rats were sacrificed.

Results: All three treatments (HBO, exercise, and both combined) significantly promoted the expression of the osteoblast-related gene and oxidative metabolism-related gene (PGC-1α). They also exerted significant inhibitory effects on the osteoclast-related mRNA expression (RANKL) and bone resorption marker CTX-I. Additionally, exercise and the combination exercise-HBO treatment increased serum superoxide dysmutase (SOD) and sclerostin expression. No significant between-group difference was observed.

Conclusions: Hyperbaric oxygen, exercise, and the combination ameliorated bone microarchitecture deterioration and ovariectomy-induced bone loss in rats, and these inhibitory effects may be associated with the increased SOD and up-regulated PGC-1α.

查看原文本刊更多论文

高压氧和跑步机运动部分预防了去卵巢大鼠的骨丢失和骨微结构恶化。

引言：先前的研究已经证明了平板运动（EX）对骨质疏松症的有益作用，以及高压氧（HBO）对体外成骨细胞和破骨细胞形成的有益作用。我们研究了HBO以及HBO和EX联合用药对去卵巢大鼠骨质疏松症的影响。方法：将43只3个月大的雌性Sprague-Dawley大鼠随机分为5组（n=8）：假对照组（对照组）；卵巢切除术组；踏车运动治疗组；HBO治疗组；HBO联合踏车运动组。HBO暴露为203 kPa，85-90%O₂, 90分钟，运动方案为20 m·min⁻cco，40分钟·天cco，5°斜率。两种治疗方法每天给药一次，每周5天，持续12周，直到处死大鼠。结果：三种治疗（HBO、运动和两者联合）均显著促进了成骨细胞相关基因和氧化代谢相关基因（PGC-1α）的表达。它们还对破骨细胞相关mRNA表达（RANKL）和骨吸收标志物CTX-I产生显著的抑制作用。此外，运动和联合运动HBO治疗增加了血清超氧化物歧化酶（SOD）和硬化素的表达。组间无显著差异。结论：高压氧、运动和联合应用可改善大鼠骨微结构恶化和卵巢切除术引起的骨丢失，这些抑制作用可能与SOD增加和PGC-1α上调有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Diving and hyperbaric medicine 医学-公共卫生、环境卫生与职业卫生

CiteScore

1.70

自引率

22.20%

发文量

审稿时长

>12 weeks

期刊介绍： Diving and Hyperbaric Medicine (DHM) is the combined journal of the South Pacific Underwater Medicine Society (SPUMS) and the European Underwater and Baromedical Society (EUBS). It seeks to publish papers of high quality on all aspects of diving and hyperbaric medicine of interest to diving medical professionals, physicians of all specialties, scientists, members of the diving and hyperbaric industries, and divers. Manuscripts must be offered exclusively to Diving and Hyperbaric Medicine, unless clearly authenticated copyright exemption accompaniesthe manuscript. All manuscripts will be subject to peer review. Accepted contributions will also be subject to editing.