Expression of FGF-23 and FGFR1 is increased in uremic rat skin.

IF 1.1 4区医学 Q3 UROLOGY & NEPHROLOGY

Clinical nephrology Pub Date : 2023-09-01 DOI:10.5414/CN111082

Sema Tulay Koz, Ozge Ozkaynak, Suleyman Koz, Huseyin Aydin, O Fahrettin Goze

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引用次数: 0

Abstract

Purpose: Alterations in skin structure and function are very common in uremic patients, but still there is no unifying hypothesis for uremic skin disorders. Fibroblast growth factor-23 (FGF-23) deficiency has been linked to skin disorders in non-uremic animals. We aimed to study alterations in FGF-23 and fibroblast growth factor-23 receptor 1 (FGFR1) expression in uremic rat skins.

Material and methods: Wistar albino rats were divided into two groups: sham group (SG, n = 8) and uremic group (UG, n = 8). Uremia was induced by reduction of the total kidney mass in the UG. Animals were sacrificed after 14 weeks of the follow-up.

Results: Serum creatinine and blood urea nitrogen levels in the UG increased significantly, compared to the SG, at the end of the experiment (0.69 ± 0.08 vs. 0.3 ± 0.04 Mann-Whitney U test (MWU), p = 0,003 and 55.2 ± 8.9 vs. 29.6 ± 6.8 MWU, p = 0.002, respectively). Serum FGF-23 level in the UG was increased non-significantly, compared to the SG (53.5 ± 20.9 vs. 37.2 ± 9.7 MWU, p = 0.072), whereas serum 1,25(OH)₂D₃ level was significantly lower in the UG (149.4 ± 33.5 vs. 213.8 ± 43.8 MWU, p < 0.05). Expression of FGF-23 in UG skins, assessed by western blot, was significantly higher than that in the SG (186.3 ± 16.8 vs. 148.9 ± 25.9, MWU, p < 0.01). FGFR1 expression was increased in almost all parts of the uremic skin. Receptor expression was most dense at the epidermis and hair follicles. Normal skin appendages and cells either expressed no receptor, or expressed it very weakly.

Conclusion: This study shows increased FGF-23 levels and FGFR1 expression in uremic rat skins. It deserves further study to fully place this finding in the pathophysiology and clinical picture of uremic skin diseases.

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在尿毒症大鼠皮肤中，FGF-23和FGFR1的表达增加。

目的:皮肤结构和功能的改变在尿毒症患者中很常见，但对尿毒症皮肤病仍没有统一的假设。成纤维细胞生长因子-23 (FGF-23)缺乏与非尿毒症动物的皮肤疾病有关。我们旨在研究尿毒症大鼠皮肤中FGF-23和成纤维细胞生长因子-23受体1 (FGFR1)表达的变化。材料与方法:将Wistar白化大鼠分为两组:假手术组(SG, n = 8)和尿毒症组(UG, n = 8)， UG通过减少肾脏总质量诱导尿毒症。动物在随访14周后被处死。结果:实验结束时，UG组血清肌酐和尿素氮水平较SG组显著升高(Mann-Whitney U检验(MWU)，分别为0.69±0.08比0.3±0.04,p = 0.003和55.2±8.9比29.6±6.8,p = 0.002)。UG组血清FGF-23水平较SG组无显著升高(53.5±20.9∶37.2±9.7 MWU, p = 0.072)，而UG组血清1,25(OH)2D3水平较SG组显著降低(149.4±33.5∶213.8±43.8 MWU, p < 0.05)。western blot检测UG皮肤中FGF-23的表达水平显著高于SG(186.3±16.8比148.9±25.9,MWU, p < 0.01)。几乎所有尿毒症皮肤的FGFR1表达均升高。受体在表皮和毛囊的表达最为密集。正常的皮肤附属物和细胞要么不表达受体，要么表达非常弱。结论:本研究显示尿毒症大鼠皮肤中FGF-23水平和FGFR1表达升高。在尿毒症皮肤病的病理生理学和临床表现方面，这一发现值得进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical nephrology 医学-泌尿学与肾脏学

CiteScore

2.10

自引率

9.10%

发文量

138

审稿时长

4-8 weeks

期刊介绍： Clinical Nephrology appears monthly and publishes manuscripts containing original material with emphasis on the following topics: prophylaxis, pathophysiology, immunology, diagnosis, therapy, experimental approaches and dialysis and transplantation.