Protein engineering of pores for separation, sensing, and sequencing.

IF 9 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Laxmicharan Samineni, Bibek Acharya, Harekrushna Behera, Hyeonji Oh, Manish Kumar, Ratul Chowdhury
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引用次数: 0

Abstract

Proteins are critical to cellular function and survival. They are complex molecules with precise structures and chemistries, which allow them to serve diverse functions for maintaining overall cell homeostasis. Since the discovery of the first enzyme in 1833, a gamut of advanced experimental and computational tools has been developed and deployed for understanding protein structure and function. Recent studies have demonstrated the ability to redesign/alter natural proteins for applications in industrial processes of interest and to make customized, novel synthetic proteins in the laboratory through protein engineering. We comprehensively review the successes in engineering pore-forming proteins and correlate the amino acid-level biochemistry of different pore modification strategies to the intended applications limited to nucleotide/peptide sequencing, single-molecule sensing, and precise molecular separations.

用于分离、传感和测序的孔隙蛋白质工程。
蛋白质对细胞功能和存活至关重要。它们是复杂的分子,具有精确的结构和化学成分,这使它们能够发挥各种功能,维持细胞的整体稳态。自1833年发现第一种酶以来,一系列先进的实验和计算工具被开发出来,用于理解蛋白质的结构和功能。最近的研究已经证明了重新设计/改变天然蛋白质用于工业过程的能力,以及通过蛋白质工程在实验室中制造定制的新型合成蛋白质的能力。我们全面回顾了工程孔形成蛋白的成功,并将不同孔修饰策略的氨基酸水平生物化学与仅限于核苷酸/肽测序,单分子传感和精确分子分离的预期应用相关联。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell Systems
Cell Systems Medicine-Pathology and Forensic Medicine
CiteScore
16.50
自引率
1.10%
发文量
84
审稿时长
42 days
期刊介绍: In 2015, Cell Systems was founded as a platform within Cell Press to showcase innovative research in systems biology. Our primary goal is to investigate complex biological phenomena that cannot be simply explained by basic mathematical principles. While the physical sciences have long successfully tackled such challenges, we have discovered that our most impactful publications often employ quantitative, inference-based methodologies borrowed from the fields of physics, engineering, mathematics, and computer science. We are committed to providing a home for elegant research that addresses fundamental questions in systems biology.
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