Filtration of environmentally sourced aquatic media impacts laboratory-colonised Aedes albopictus early development and adult bacteriome composition

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY
Chasen D. Griffin, Danya E. Weber, Priscilla Seabourn, Lorraine K. Waianuhea, Matthew C. I. Medeiros
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引用次数: 0

Abstract

Microorganisms form close associations with metazoan hosts forming symbiotic communities, known as microbiomes, that modulate host physiological processes. Mosquitoes are of special interest in exploring microbe-modulated host processes due to their oversized impact on human health. However, most mosquito work is done under controlled laboratory conditions where natural microbiomes are not present and inferences from these studies may not extend to natural populations. Here we attempt to assemble a wild-resembling bacteriome under laboratory conditions in an established laboratory colony of Aedes albopictus using aquatic media from environmentally-exposed and differentially filtered larval habitats. While we did not successfully replicate a wild bacteriome using these filtrations, we show that these manipulations alter the bacteriomes of mosquitoes, generating a unique composition not seen in wild populations collected from and near our source water or in our laboratory colony. We also demonstrate that our filtration regimens impact larval development times, as well as impact adult survival on different carbohydrate diets.

Abstract Image

环境源水生媒介的过滤影响实验室定殖白纹伊蚊的早期发育和成虫细菌组组成。
微生物与后生动物宿主形成密切联系,形成共生群落,称为微生物群,调节宿主的生理过程。蚊子对探索微生物调节的宿主过程特别感兴趣,因为它们对人类健康的影响过大。然而,大多数蚊子的工作都是在受控的实验室条件下进行的,那里不存在自然微生物群,这些研究的推论可能不会延伸到自然种群。在这里,我们试图在实验室条件下,使用来自环境暴露和不同过滤幼虫栖息地的水生介质,在白纹伊蚊的既定实验室菌落中组装一个类似野生的细菌组。虽然我们没有使用这些过滤成功复制野生菌群,但我们表明,这些操作改变了蚊子的菌群,产生了一种独特的成分,这种成分在从我们的水源水及其附近或实验室菌落中采集的野生种群中是看不到的。我们还证明,我们的过滤方案会影响幼虫的发育时间,也会影响不同碳水化合物饮食的成虫存活率。
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来源期刊
Medical and Veterinary Entomology
Medical and Veterinary Entomology 农林科学-昆虫学
CiteScore
3.70
自引率
5.30%
发文量
65
审稿时长
12-24 weeks
期刊介绍: Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.
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