Comparison of KRAS gene in circulating tumor DNA levels vs histological grading of colorectal cancer patients through liquid biopsy.

IF 1.9 4区医学 Q3 GASTROENTEROLOGY & HEPATOLOGY

Saudi Journal of Gastroenterology Pub Date : 2023-11-01 DOI:10.4103/sjg.sjg_85_23

Hafiz Syed Mohammad Osama Jafri, Shamim Mushtaq, Saeeda Baig, Afreen Bhatty, Sabra Siraj

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引用次数: 0

Abstract

Background: To determine KRAS gene in circulating tumor DNA in comparison with histological grading through liquid biopsy in colorectal cancer patients.

Methods: This dual-centered cross-sectional study included 73 diagnosed patients of colorectal cancer at different grading levels [Grade I, well differentiated (n = 7, 9.5%); Grade II, moderately differentiated (n = 14,18.9%); and Grade III, poorly differentiated (n = 52, 70%)]. Blood was collected, and plasma was separated. ctDNA was extracted, using magnetic bead-based technique (MagMAX Cell-Free DNA kit). KRAS gene was quantified through qPCR. STRING database was used to find KRAS interactomes.

Results: Mean threshold cycle (CT value) of KRAS gene in Grade III samples showed significantly higher (P = 0.001) levels of ctDNA (2.7 ± 1.14) compared with Grade II and Grade I (3.1 ± 0.68, 2.3 ± 0.60), respectively. Grading characterization showed that rectal cancer (n = 22, 42.3%) with Grade III (68.8%) was more prevalent than colon and sigmoid cancer (n = 19, 36.5%, n = 11, 21%, respectively). STRING database showed 10 functional genes interacting with KRAS expressed as gene/proteins.

Conclusion: Liquid biopsy can be used to detect ctDNA in plasma of CRC patients and enabled to detect the KRAS gene by qPCR. The technique being less invasive and cost-effective is convenient for multiple biopsies in different cancers.

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结直肠癌患者循环肿瘤DNA中KRAS基因水平与液体活检组织学分级的比较

背景:通过液体活检检测结直肠癌患者循环肿瘤DNA中的KRAS基因并与组织学分级进行比较。方法:本双中心横断面研究纳入73例不同分级的结直肠癌确诊患者[I级，高分化(n = 7, 9.5%);II级，中度分化(n = 14,18.9%);III级，低分化(n = 52,70%)]。采集了血液，分离了血浆。利用磁珠技术(MagMAX Cell-Free DNA试剂盒)提取ctDNA。采用qPCR方法对KRAS基因进行定量分析。使用STRING数据库查找KRAS交互组。结果:三级患者KRAS基因平均阈值(CT值)ctDNA水平(2.7±1.14)明显高于二级患者(3.1±0.68)、一级患者(2.3±0.60)(P = 0.001)。分级特征显示，III级直肠癌(n = 22,42.3%)(68.8%)比结肠癌和乙状结肠癌(n = 19,36.5%， n = 11,21%)更为普遍。STRING数据库显示10个与KRAS相互作用的功能基因以基因/蛋白的形式表达。结论:液体活检可检测CRC患者血浆中ctDNA, qPCR可检测KRAS基因。该技术侵入性小，成本效益高，便于对不同癌症进行多次活检。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Saudi Journal of Gastroenterology GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

4.40

自引率

3.70%

发文量

审稿时长

28 weeks

期刊介绍： The Saudi Journal of Gastroenterology (SJG) is an open access peer-reviewed publication. Authors are invited to submit articles in the field of gastroenterology, hepatology and nutrition, with a wide spectrum of coverage including basic science, epidemiology, diagnostics, therapeutics, public health, and standards of health care in relation to the concerned specialty. Review articles are usually by invitation. However review articles of current interest and a high standard of scientific value could also be considered for publication.