Nitrous Oxide-Induced Metaphase Arrest: A Technique for Somatic Chromosome Analysis.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2023-01-01 DOI:10.1007/978-1-0716-3226-0_6

Patrice S Albert, James A Birchler

引用次数: 0

Abstract

Procedures to arrest metaphase chromosomes are used for determining chromosome numbers, chromosomal aberrations, and natural chromosome variation, as well as chromosome sorting. Here is described a technique of nitrous oxide gas treatment of freshly harvested root tips that is highly effective at producing an excellent mitotic index together with well-spread chromosomes. The details of the treatment and equipment used are provided. The metaphase spreads can be used directly for determining chromosome numbers or for in situ hybridization to reveal chromosomal features.

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氧化亚氮诱导的中期阻滞:一种体细胞染色体分析技术。

捕获中期染色体的程序用于确定染色体数目、染色体畸变和自然染色体变异，以及染色体分选。这里描述了一种技术的氧化亚氮气体处理新收获的根尖，是非常有效的产生一个优秀的有丝分裂指数和良好的分布染色体。提供了治疗的细节和使用的设备。中期扩散可直接用于确定染色体数目或原位杂交以揭示染色体特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.

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