LncRNA FOXD3-AS1/miR-128-3p axis-mediated IGF2BP3 in glioma stimulates cancer angiogenesis and progression.

IF 1.5 4区医学 Q4 NEUROSCIENCES

Folia neuropathologica Pub Date : 2023-01-01 DOI:10.5114/fn.2023.126862

Hongxin Zhao, Yuyu Wang, Chuandong Liang, Mingxiang Xie

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引用次数: 0

Abstract

Introduction: The aim of the study was to research the mechanism by which IGF2BP3 regulates glioma progression as well as its upstream regulatory axis.

Material and methods: The researched mRNA was determined using differential expression analysis based on bioinformatics data, and its upstream miRNAs and lncRNAs were predicted. Interaction between genes we researched was identified by dual-luciferase method. The viability, migration, invasion and angiogenesis of glioma were measured with MTT, colony formation, Transwell and Matrigel tube formation experiments, respectively. The mRNA expression of each gene was tested with qRT-PCR. IGF2BP3 level was determined via western blot and immunohistochemistry. Subcellular fractionation of FOXD3-AS1 was tested with fluorescence in situ hybridization. In vivo tumorigenesis assay was conducted on nude mice.

Results: IGF2BP3 high level in glioma cells correlated with patient's prognosis. Downregulation of IGF2BP3 restrained proliferation, migration, invasion and angiogenesis in glioma cells both in vitro and in vivo. There was a binding relationship between IGF2BP3 and miR-128-3p. Besides, FOXD3-AS1 as a sponge of miR-128-3p was located mainly in cytoplasm. Additionally, FOXD3-AS1 facilitated IGF2BP3 level via sponging miR-128-3p to stimulate glioma angiogenesis.

Conclusions: FOXD3-AS1 was a sponge of miR-128-3p through upregulating IGF2BP3 in glioma. Our findings shed light on diagnosis and treatment of glioma.

查看原文本刊更多论文

LncRNA FOXD3-AS1/miR-128-3p轴介导的IGF2BP3刺激胶质瘤血管生成和进展。

本研究旨在研究IGF2BP3调控胶质瘤进展的机制及其上游调控轴。材料与方法:采用基于生物信息学数据的差异表达分析确定研究mRNA，并预测其上游mirna和lncrna。用双荧光素酶法鉴定了所研究基因间的相互作用。采用MTT、集落形成、Transwell和Matrigel试管形成实验分别测定胶质瘤的生存能力、迁移能力、侵袭能力和血管生成能力。采用qRT-PCR检测各基因mRNA表达量。western blot和免疫组化检测IGF2BP3水平。荧光原位杂交检测FOXD3-AS1的亚细胞分离。裸鼠进行体内肿瘤发生实验。结果:胶质瘤细胞中IGF2BP3水平高与患者预后相关。IGF2BP3的下调对胶质瘤细胞的增殖、迁移、侵袭和血管生成均有抑制作用。IGF2BP3与miR-128-3p之间存在结合关系。此外，FOXD3-AS1作为miR-128-3p的海绵主要位于细胞质中。此外，FOXD3-AS1通过海绵miR-128-3p促进IGF2BP3水平，刺激胶质瘤血管生成。结论:FOXD3-AS1在胶质瘤中通过上调IGF2BP3成为miR-128-3p的海绵。我们的发现为胶质瘤的诊断和治疗提供了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia neuropathologica 医学-病理学

CiteScore

2.50

自引率

5.00%

发文量

审稿时长

>12 weeks

期刊介绍： Folia Neuropathologica is an official journal of the Mossakowski Medical Research Centre Polish Academy of Sciences and the Polish Association of Neuropathologists. The journal publishes original articles and reviews that deal with all aspects of clinical and experimental neuropathology and related fields of neuroscience research. The scope of journal includes surgical and experimental pathomorphology, ultrastructure, immunohistochemistry, biochemistry and molecular biology of the nervous tissue. Papers on surgical neuropathology and neuroimaging are also welcome. The reports in other fields relevant to the understanding of human neuropathology might be considered.