ADAMTS8 inhibits glioma development in vitro and in vivo.

IF 1.5 4区医学 Q4 NEUROSCIENCES

Folia neuropathologica Pub Date : 2023-01-01 DOI:10.5114/fn.2023.129380

Baosheng Zhou, Yong Liu, Guangshuo Ma, Xiuyu Wang, Binge Chang, Hongwei Lu, Xuequan Feng

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引用次数: 0

Abstract

Introduction: In recent years, novel RNAs have been revealed to be regulators in glioma. ADAMTS8 has been reported to be reduced in brain tumours. In this study, we aimed to explore the role of ADAMTS8 in glioma.

Material and methods: Online bioinformatic tools, Gepia and Chinese Glioma Genome Atlas database (CGGA) were used to analyse the differential expression of ADAMTS8, overall survival and disease-free survival rates and the correlations between ADAMTS8 and matrix metallopeptidases (MMP2 and MMP9) in glioma. RT-qPCR and western blot experiments were performed to measure the mRNA and protein expression. ADAMTS8 expression was regulated in cells through transfection. Thereafter, the effect of ADAMTS8 on cells was investigated through the cell viability, apoptosis and transwell experiments. The epithelial-mesenchymal transition (EMT)-related proteins and also MMP2 and MMP9 were examined. The subcutaneous tumour model was established to validate the suppressive role of ADAMTS8 in tumour growth.

Results: ADAMTS8 expression was reduced in glioma tissues and cells. Higher expression of ADAMTS8 was correlated with higher survival rates. ADAMTS8 was correlated with MMP2 and MMP9 in glioma tissues. In glioma cells, overexpression of ADAMTS8 could inhibit the viability, invasion, migration and EMT, and MMP2 and MMP9, but promote the apoptosis of cells. The upregulation of ADAMTS8 could inhibit the tumour growth in vivo.

Conclusions: ADAMTS8 was inhibited in glioma and the higher expression of ADAMTS8 might be related to better prognosis among glioma patients. Overexpression of ADAMTS8 inhibited the development of glioma in vitro and in vivo.

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ADAMTS8在体外和体内抑制胶质瘤的发展。

近年来，新的rna被发现在胶质瘤中起调节作用。据报道，ADAMTS8在脑肿瘤中减少。在这项研究中，我们旨在探讨ADAMTS8在胶质瘤中的作用。材料和方法:利用在线生物信息学工具Gepia和中国胶质瘤基因组图谱数据库(CGGA)分析胶质瘤中ADAMTS8的差异表达、总生存率和无病生存率以及ADAMTS8与基质金属肽酶(MMP2和MMP9)的相关性。RT-qPCR和western blot检测mRNA和蛋白的表达。转染后，ADAMTS8在细胞中表达得到调控。随后，通过细胞活力、凋亡和transwell实验研究ADAMTS8对细胞的影响。检测上皮-间质转化(epithelial-mesenchymal transition, EMT)相关蛋白以及MMP2和MMP9。建立皮下肿瘤模型，验证ADAMTS8对肿瘤生长的抑制作用。结果:ADAMTS8在胶质瘤组织和细胞中表达降低。ADAMTS8的高表达与较高的存活率相关。在胶质瘤组织中，ADAMTS8与MMP2、MMP9相关。在胶质瘤细胞中，过表达ADAMTS8可抑制细胞活力、侵袭、迁移和EMT，抑制MMP2和MMP9，但促进细胞凋亡。上调ADAMTS8可抑制肿瘤在体内的生长。结论:ADAMTS8在胶质瘤中受到抑制，ADAMTS8的高表达可能与胶质瘤患者预后较好有关。在体内和体外，过表达ADAMTS8抑制胶质瘤的发生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia neuropathologica 医学-病理学

CiteScore

2.50

自引率

5.00%

发文量

审稿时长

>12 weeks

期刊介绍： Folia Neuropathologica is an official journal of the Mossakowski Medical Research Centre Polish Academy of Sciences and the Polish Association of Neuropathologists. The journal publishes original articles and reviews that deal with all aspects of clinical and experimental neuropathology and related fields of neuroscience research. The scope of journal includes surgical and experimental pathomorphology, ultrastructure, immunohistochemistry, biochemistry and molecular biology of the nervous tissue. Papers on surgical neuropathology and neuroimaging are also welcome. The reports in other fields relevant to the understanding of human neuropathology might be considered.