Kami Ahmad, Steven Henikoff, Srinivas Ramachandran
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引用次数: 0
Abstract
Gene regulation arises out of dynamic competition between nucleosomes, transcription factors, and other chromatin proteins for the opportunity to bind genomic DNA. The timescales of nucleosome assembly and binding of factors to DNA determine the outcomes of this competition at any given locus. Here, we review how these properties of chromatin proteins and the interplay between the dynamics of different factors are critical for gene regulation. We discuss how molecular structures of large chromatin-associated complexes, kinetic measurements, and high resolution mapping of protein-DNA complexes in vivo set the boundary conditions for chromatin dynamics, leading to models of how the steady state behaviors of regulatory elements arise.
基因调控源于核小体、转录因子和其他染色质蛋白之间为争夺与基因组 DNA 结合的机会而展开的动态竞争。核小体组装和因子与 DNA 结合的时间尺度决定了任何给定基因座上这种竞争的结果。在这里,我们将回顾染色质蛋白的这些特性以及不同因子动态之间的相互作用是如何对基因调控起到关键作用的。我们将讨论大型染色质相关复合物的分子结构、动力学测量以及体内蛋白质-DNA 复合物的高分辨率图谱如何为染色质动力学设定边界条件,从而建立调控因子稳态行为的模型。
期刊介绍:
The Annual Review of Biochemistry, in publication since 1932, sets the standard for review articles in biological chemistry and molecular biology. Since its inception, these volumes have served as an indispensable resource for both the practicing biochemist and students of biochemistry.