Effects of Capsular Polysaccharide amount on Pneumococcal-Host interactions.

IF 6.7 1区 医学 Q1 Immunology and Microbiology
PLoS Pathogens Pub Date : 2023-08-04 eCollection Date: 2023-08-01 DOI:10.1371/journal.ppat.1011509
Jiaqi Zhu, Annie R Abruzzo, Cindy Wu, Gavyn Chern Wei Bee, Alejandro Pironti, Gregory Putzel, Surya D Aggarwal, Hannes Eichner, Jeffrey N Weiser
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引用次数: 0

Abstract

Among the many oral streptococci, Streptococcus pneumoniae (Spn) stands out for the capacity of encapsulated strains to cause invasive infection. Spread beyond upper airways, however, is a biological dead end for the organism, raising the question of the benefits of expending energy to coat its surface in a thick layer of capsular polysaccharide (CPS). In this study, we compare mutants of two serotypes expressing different amounts of CPS and test these in murine models of colonization, invasion infection and transmission. Our analysis of the effect of CPS amount shows that Spn expresses a capsule of sufficient thickness to shield its surface from the deposition of complement and binding of antibody to underlying epitopes. While effective shielding is permissive for invasive infection, its primary contribution to the organism appears to be in the dynamics of colonization. A thicker capsule increases bacterial retention in the nasopharynx, the first event in colonization, and also impedes IL-17-dependent clearance during late colonization. Enhanced colonization is associated with increased opportunity for host-to-host transmission. Additionally, we document substantial differences in CPS amount among clinical isolates of three common serotypes. Together, our findings show that CPS amount is highly variable among Spn and could be an independent determinant affecting host interactions.

Abstract Image

Abstract Image

Abstract Image

荚膜多糖含量对肺炎球菌与宿主相互作用的影响。
在许多口腔链球菌中,肺炎链球菌(Spn)因其包封菌株引起侵袭性感染的能力而脱颖而出。然而,扩散到上呼吸道之外是生物的死胡同,这就提出了消耗能量在其表面覆盖一层厚厚的荚膜多糖(CPS)的好处的问题。在这项研究中,我们比较了表达不同数量CPS的两种血清型突变体,并在小鼠模型中测试了这些突变体的定植、侵袭感染和传播。我们对CPS量影响的分析表明,Spn表达了一个足够厚度的胶囊,以保护其表面不受补体沉积和抗体与底层表位结合的影响。虽然有效的屏蔽对侵袭性感染是允许的,但它对生物体的主要贡献似乎是定植的动态。较厚的胶囊增加了细菌在鼻咽的滞留,这是定植的第一个事件,并且也阻碍了定植后期il -17依赖的清除。定植的增强与宿主间传播的机会增加有关。此外,我们记录了三种常见血清型临床分离株中CPS量的实质性差异。总之,我们的研究结果表明,CPS的数量在Spn中变化很大,可能是影响宿主相互作用的独立决定因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
PLoS Pathogens
PLoS Pathogens 生物-病毒学
CiteScore
11.40
自引率
3.00%
发文量
598
审稿时长
2 months
期刊介绍: Bacteria, fungi, parasites, prions and viruses cause a plethora of diseases that have important medical, agricultural, and economic consequences. Moreover, the study of microbes continues to provide novel insights into such fundamental processes as the molecular basis of cellular and organismal function.
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