Artificial cells eavesdropping on HepG2 cells.

IF 3.6 3区生物学 Q1 BIOLOGY

Interface Focus Pub Date : 2023-08-11 eCollection Date: 2023-10-06 DOI:10.1098/rsfs.2023.0007

Isabella Nymann Westensee, Brigitte Städler

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Abstract

Cellular communication is a fundamental feature to ensure the survival of cellular assemblies, such as multicellular tissue, via coordinated adaption to changes in their surroundings. Consequently, the development of integrated semi-synthetic systems consisting of artificial cells (ACs) and mammalian cells requires feedback-based interactions. Here, we illustrate that ACs can eavesdrop on HepG2 cells focusing on the activity of cytochrome P450 1A2 (CYP1A2), an enzyme from the cytochrome P450 enzyme family. Specifically, d-cysteine is sent as a signal from the ACs via the triggered reduction of disulfide bonds. Simultaneously, HepG2 cells enzymatically convert 2-cyano-6-methoxybenzothiazole into 2-cyano-6-hydroxybenzothiazole that is released in the extracellular space. d-Cysteine and 2-cyano-6-hydroxybenzothiazole react to form d-luciferin. The ACs respond to this signal by converting d-luciferin into luminescence due to the presence of encapsulated luciferase in the ACs. As a result, the ACs can eavesdrop on the mammalian cells to evaluate the level of hepatic CYP1A2 function.

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偷听HepG2细胞的人工细胞。

细胞通讯是通过协调适应周围环境的变化来确保细胞组件（如多细胞组织）生存的基本特征。因此，开发由人工细胞（AC）和哺乳动物细胞组成的集成半合成系统需要基于反馈的相互作用。在这里，我们说明了AC可以窃听HepG2细胞，重点是细胞色素P450 1A2（CYP1A2）的活性，CYP1A2是细胞色素P450酶家族的一种酶。具体而言，d-半胱氨酸作为信号通过二硫键的触发还原从AC发出。同时，HepG2细胞酶促将2-氰基-6-甲氧基苯并噻唑转化为2-氰基6-羟基苯并噻唑，并在细胞外空间释放。d-半胱氨酸和2-氰基-6-羟基苯并噻唑反应生成d-荧光素。由于AC中存在包封的荧光素酶，AC通过将d-荧光素转化为发光来响应该信号。因此，AC可以窃听哺乳动物细胞以评估肝脏CYP1A2功能的水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Interface Focus BIOLOGY-

CiteScore

9.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Each Interface Focus themed issue is devoted to a particular subject at the interface of the physical and life sciences. Formed of high-quality articles, they aim to facilitate cross-disciplinary research across this traditional divide by acting as a forum accessible to all. Topics may be newly emerging areas of research or dynamic aspects of more established fields. Organisers of each Interface Focus are strongly encouraged to contextualise the journal within their chosen subject.