A self-cleavable ULP1-SUMO fusion strategy for enhanced soluble protein expression and one-step purification in Escherichia coli

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DOI:10.1016/j.pep.2026.106892
作者：Changbin Jin ,?Xiaohu Zhang ,?Zhibo Yang ,?Wei Yuan ,?Hao Shi ,?Jingru Zhu ,?Yueping Zhang
文献类型：期刊论文
补充材料：只需要正文

Elsevier

Abstract：The Escherichia coli (E. coli) expression system is widely used for recombinant protein production; however, protein insolubility remains a major challenge. Although fusion tags can enhance

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